Protein Info for ABID97_RS23805 in Variovorax sp. OAS795
Annotation: amidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 95% identity to vap:Vapar_4356)Predicted SEED Role
"Amidase (EC 3.5.1.4)" (EC 3.5.1.4)
MetaCyc Pathways
- L-glutamate and L-glutamine biosynthesis (7/7 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of acrylonitrile degradation (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- acrylonitrile degradation I (2/2 steps found)
- indole-3-acetate biosynthesis IV (bacteria) (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- L-arginine degradation X (arginine monooxygenase pathway) (2/3 steps found)
- indole-3-acetate biosynthesis III (bacteria) (1/2 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- indole-3-acetate biosynthesis II (5/12 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Aminoacyl-tRNA biosynthesis
- Arginine and proline metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Cyanoamino acid metabolism
- Glutamate metabolism
- Phenylalanine metabolism
- Styrene degradation
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.4, 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 3.5.1.4 or 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (471 amino acids)
>ABID97_RS23805 amidase (Variovorax sp. OAS795) MSADVSSSSLNDLSARELSAAYREKRLSPVEVAQAVIAHIERWEPQLQATWLFRPEAALE QARASEARWQRGEALGPLDGVPATIKENIATRGDPMPAGTAAVDLKPAAADAPPAARLKE AGAVVVSKTTMPDYGMLSSGLSSFHKLARNPWDLRKTPGGSSAGAGAAAAAGYGPLHIGT DIGGSLRLPASWCGIFTLKPSLGRIPIDPPYMGRAAGPMTRSVGDAALMMQVLSRPDARD SMSLPPQDIDWANFDVSPDHLRGLRIGLLLDAGCGLAVEPEIQAAVEHAARLFEKAGAVV ETMQPFMSQTMLDGMDHLWRMRSLVDMKALRPDQRAKVLPYIREWAESAAEFSGEHVFRG FSQFHATRVAAVQACARFDYVISPVSPNMPAAAEAPSPTNDPLRPLEHIGFTVPFNMSEQ PASSVNCGYSADGLPIGLQIAGKRFDDLGVLRVSRAFEQIREPQRPWPVGP