Protein Info for ABID97_RS23670 in Variovorax sp. OAS795

Annotation: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 751 PF13328: HD_4" amino acids 51 to 200 (150 residues), 168.9 bits, see alignment E=2.5e-53 TIGR00691: RelA/SpoT family protein" amino acids 51 to 743 (693 residues), 701.5 bits, see alignment E=6e-215 PF01966: HD" amino acids 70 to 169 (100 residues), 34.5 bits, see alignment E=7.7e-12 PF04607: RelA_SpoT" amino acids 260 to 369 (110 residues), 125.4 bits, see alignment E=4.5e-40 PF02824: TGS" amino acids 413 to 472 (60 residues), 75.4 bits, see alignment 1e-24 PF19296: RelA_AH_RIS" amino acids 486 to 640 (155 residues), 27.4 bits, see alignment E=1.2e-09 PF13291: ACT_4" amino acids 669 to 746 (78 residues), 46.5 bits, see alignment E=1.7e-15 PF01842: ACT" amino acids 675 to 736 (62 residues), 29.7 bits, see alignment 1.6e-10

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 99% identity to vap:Vapar_4331)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (751 amino acids)

>ABID97_RS23670 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Variovorax sp. OAS795)
MSAVAKHQSHAPSGTPKPSPAALNAAAASFAALTARLDYLSPEDTELVRRAYRFADEAHL
GQLRNSGEPYITHPIAVAAQCAEWKLDAQALMAALLHDAIEDCGVTKPELIERFGAPVAE
LVDGLTKLDKLQFNTREENQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDDAPREKW
ARISRETLEIYAPIAHRLGLNQTYRELQELSFRHLKPWRYATLSKAVARARGRRRDLIQK
VQREVESAFANANMTLRIAGREKTLYSIYRKMEEKHLSFAQVTDIYGFRLIVPNVIACYT
GLGILHQMYKPLPGKFKDHIAIAKLNGYQSLHTTLVGPAGVNVEFQLRTEAMHVVAESGV
AAHWLYKAAEPNAASNDRLGTKWLQSLLDIQDETRDAAEFWDHVKVDLFPDAVYVFTPKS
QILALPRGATVVDFAYAIHSNIGDHTSAARINGDQVPLRTELKNGDVVEVITAPVSTPNP
AWLGFVRTGRARSKIRHYLKTLAHAESEGLGEKLLAQALRAEGLSKLPADDAEHQPLWEK
LLRFTGNRTRAELLTDIGLGKRIASIVAKRLVAMLSELGERPDALLLSRERFISHETISQ
GAVTLDGSENSSVRFALCCRPIPGDEIVGYLGHGEGLVVHTESCGVGQRLRHKDSERFFA
VEWADEPTRAFETGVVITVRNDKGVLARVAATLADAEADITHVEMADETPQDSTDLRFVI
AVRDRTHLDAVLRAARRTASVLTAARTVPAP