Protein Info for ABID97_RS23485 in Variovorax sp. OAS795

Annotation: bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00120: glutamate N-acetyltransferase/amino-acid acetyltransferase
  • amino acids 15 to 409 (395 residues), 399.6 bits, E=7.2e-124
• PF01960: ArgJ
  • amino acids 22 to 409 (388 residues), 475.3 bits, E=5.7e-147

Best Hits

Swiss-Prot: 69% identical to ARGJ_RALSO: Arginine biosynthesis bifunctional protein ArgJ (argJ) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00620, glutamate N-acetyltransferase / amino-acid N-acetyltransferase [EC: 2.3.1.1 2.3.1.35] (inferred from 96% identity to vpe:Varpa_4843)

Predicted SEED Role

"Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1)" in subsystem Arginine Biosynthesis extended (EC 2.3.1.1, EC 2.3.1.35)

MetaCyc Pathways

• superpathway of arginine and polyamine biosynthesis (16/17 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-ornithine biosynthesis I (5/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.1

Use Curated BLAST to search for 2.3.1.1 or 2.3.1.35

Protein Sequence (409 amino acids)

>ABID97_RS23485 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ (Variovorax sp. OAS795)
MPVNLSAPDPAALFAVPGVRIGVAEAGVRKANRKDLTVVLIDEGSAVGGVFTQNRFCAAP
VQVCRDHLAANYGIRAMVINTGNANAGTGEDGLMRTRSTCIALARHLEIAPEQILPFSTG
VIMEPLPVDRIEAGLPAALADASENHWARAAEGIMTTDTIPKAFSQQAEVGGATVTITGI
SKGAGMIRPNMATMLGFMATDAKVDPALIQPLAKLLADASFNRVTIDGDTSTNDSFVVIA
TQKAAHAPITSLDSADGQALVTAMQNVARQLAQAIVRDGEGATKFITIQVEGGRTADECK
QVAYAVAHSPLVKTAFFASDPNLGRILAAVGYAGIADLDQTGIDLFLDDVHVAVKGGRNP
SYREEDGQRVMKQSEITVRIGLGRGNASETVWTCDLSHEYVTINADYRS