Protein Info for ABID97_RS19425 in Variovorax sp. OAS795

Annotation: CoA-acylating methylmalonate-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01722: methylmalonate-semialdehyde dehydrogenase (acylating)
  • amino acids 14 to 490 (477 residues), 736.9 bits, E=4.9e-226
• PF00171: Aldedh
  • amino acids 22 to 486 (465 residues), 473.4 bits, E=3.3e-146

Best Hits

Swiss-Prot: 61% identical to MMSA_HUMAN: Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial (ALDH6A1) from Homo sapiens

KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 99% identity to vap:Vapar_3257)

MetaCyc: 80% identical to methylmalonate-semialdehyde dehydrogenase subunit (Burkholderia cepacia R34)
Methylmalonate-semialdehyde dehydrogenase (acylating). [EC: 1.2.1.27]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)

MetaCyc Pathways

• β-alanine degradation II (2/2 steps found)
• propanoyl-CoA degradation II (4/5 steps found)
• L-valine degradation I (6/8 steps found)
• β-alanine degradation I (1/2 steps found)
• 2,4-dinitrotoluene degradation (2/7 steps found)
• myo-inositol degradation I (1/7 steps found)
• myo-, chiro- and scyllo-inositol degradation (1/10 steps found)

KEGG Metabolic Maps

• Inositol phosphate metabolism
• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.27

Use Curated BLAST to search for 1.2.1.27

Protein Sequence (507 amino acids)

>ABID97_RS19425 CoA-acylating methylmalonate-semialdehyde dehydrogenase (Variovorax sp. OAS795)
MDATTTPKTQVATVKLLIGGKFVESKTSEWRDIVNPATQEVLARVPFATQAELDAAVASA
KDAFKTWRKTPLGARARIFLKYQQLIRENMGELAAILTAEQGKTLPDAEGDVFRGLEVVE
HASNIGNLQLGELANNVANGVDTYTLLQPLGVCAGITPFNFPAMIPLWMFPMAIVTGNTF
VLKPSEQDPMVTMRLCELALEAGIPPGVLNVVHGGEAVVNGICDHKDIKAISFVGSTKVG
THVYNRATLAGKRVQCMMGAKNHAIVMPDANKEQTLNALAGASFGAAGQRCMAVSVAVLV
GEARNWVPELIAKARTLKIGAGTEKGVDVGPLVSCAAFDRVNSLIERGVADGAKLELDGR
KPTVPGYEKGNFVGPTIFSGVKPGMTIYDQEVFGPVLCVADAETIDEAIELINSNPNGNG
TAIFTQSGAAARRFQEDIDVGQVGINVPIPVPVPMFSFTGSRASKLGDLGPYGKQVIMFY
TQTKTVTARWFDDSTVSHGVNTTISLK