Protein Info for ABID97_RS16020 in Variovorax sp. OAS795

Annotation: nitrite reductase large subunit NirB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 822 PF07992: Pyr_redox_2" amino acids 5 to 307 (303 residues), 188.6 bits, see alignment E=6.2e-59 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 6 to 796 (791 residues), 919.5 bits, see alignment E=1.1e-280 PF00070: Pyr_redox" amino acids 152 to 233 (82 residues), 67.2 bits, see alignment E=5.4e-22 PF18267: Rubredoxin_C" amino acids 324 to 390 (67 residues), 63.9 bits, see alignment E=3.9e-21 PF04324: Fer2_BFD" amino acids 428 to 475 (48 residues), 52.8 bits, see alignment 1.4e-17 amino acids 492 to 542 (51 residues), 44.8 bits, see alignment 4.4e-15 PF03460: NIR_SIR_ferr" amino acids 567 to 628 (62 residues), 58 bits, see alignment 2.2e-19 PF01077: NIR_SIR" amino acids 637 to 775 (139 residues), 108 bits, see alignment E=1.2e-34

Best Hits

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 87% identity to pol:Bpro_3276)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (822 amino acids)

>ABID97_RS16020 nitrite reductase large subunit NirB (Variovorax sp. OAS795)
MHKLKLVMVGNGMAGVRTLEELLKLAPDMYDITVFGAEPHPNYNRILLSPVLAGEQTVDE
IVLNSWEWYAENHITLHAGKKVVEVDRVKRIVRAVDAQGGVTEAAYDRLLMCTGSNPFML
PVPGKDLKGVIAYRDIADTDYMIETARTHKNAVVIGGGLLGLEAANGLMLRGMNVTVVHV
MPWLMERQLDDVAGKLLQKSLEDRGLKFLIGAQTQELVGNDQGRVSAIRFKDGTEVAADL
VVMAVGIRPNTELAEKMRLHCHRGIVVTDTLQTVTDARIYSVGECAAHRGIAYGLVAPLF
EQAKVAANHLAQFGIGRYLGSLTSTKLKVTGIDLFSAGEFMGGEGTEEIVMSDPFGGVYK
KLVIKDDKLVGACLYGDTVDGSWYFKLLRDGRSVHDIRDKLMFGESNIGDTGHEGHSKAA
SMPDEAEVCGCNGVTKGTICKAIKEKGLFTLDEVKKHTKASASCGSCTGLVEQILMFTAG
GDYSATPKKKAVCGCTDANHQEVREAIRKEHYLTHDEVYRNLGWRTPDGCATCRPAVNYY
LISTWPKEARDDPQSRFINERSHANIQKDGTYSVIPRMWGGHTTPDELRRIADAADKYQI
PTVKVTGGQRIDLLGVKKEDLEGVWKDIGMPSGFAYAKSLRTVKTCVGSEWCRFGTQDST
QMGKDLERALWAMYSPHKVKLAVSGCPRNCAEAGIKDVGVIGVDSGWELYVGGNGGIKTE
VAQFLVKVKTAQEVMEYSGAFLQLYREEGWYLERTVHYIGRVGLDYVKKKILEDTEGRKQ
LWERLQFALDGEPDPWFASSQASVDVRQFTPVAVVDEATQAA