Protein Info for ABID97_RS15455 in Variovorax sp. OAS795

Annotation: FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 613 PF05430: Methyltransf_30" amino acids 106 to 227 (122 residues), 102.2 bits, see alignment E=3.8e-33 PF01266: DAO" amino acids 250 to 589 (340 residues), 150.4 bits, see alignment E=2.1e-47 TIGR03197: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain" amino acids 250 to 611 (362 residues), 235.2 bits, see alignment E=7e-74 PF13450: NAD_binding_8" amino acids 252 to 283 (32 residues), 26.8 bits, see alignment (E = 1.1e-09)

Best Hits

KEGG orthology group: None (inferred from 81% identity to vap:Vapar_2983)

Predicted SEED Role

"tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase" (EC 2.1.1.61)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.61

Use Curated BLAST to search for 2.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (613 amino acids)

>ABID97_RS15455 FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC (Variovorax sp. OAS795)
MAAAPVAWRADGVPYSERFGDVYHTETGALAQSRHVFLGGCDLPGAWSGQPQWRILETGF
GLGLNFLTAWQAWRADAHRPRLLHFVSVEAHPVGPEDLLRAAGAYPELAALAKELVAQWH
GLLPGFHRLAFDEGRVLLTLCIGDVQAMLRTQRFEADSIFLDGFSPERNPAMWSPDTLKA
VSRFARQGTGIASWTYAQGVRDVLAQNGFQLERREGLPPRRDRLAGVFAPAWTVRRRGPV
SAHVERPGRCAVIGAGLAGAAVAASLARRGWQVTVLDAAERPAAGASGLPVGVLAPHVSP
DEALLSRLTRAGIRATWLELDRLLEEGREWRGSGVLERRPDGDTRVPVGWNASGPNESWP
ANAAQLADAGLPAGAPALWHARAGWVRPARLIEAWLREPGVEFLGHAPVARLSRSAGGLQ
LCDAADRLLAEADRVVVAAGHESGRFAPALPLQSVRGQVAWGRMADGLALPSTPLNGDGH
LIAHVPGDDGTLLWLAGATFDRDSTHLAPRAADTDANRARLERLHPGAAAALAPEFARGG
AQTWVGVRCASGDRRPLAGPLSEPGVWACTALGSRGLTFAALCAELLAAQWHGEPLPLPA
NLAKALGTQRTRS