Protein Info for ABID97_RS12655 in Variovorax sp. OAS795

Annotation: formylmethanofuran dehydrogenase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03121: formylmethanofuran dehydrogenase subunit A
  • amino acids 6 to 538 (533 residues), 695.5 bits, E=2e-213
• PF07969: Amidohydro_3
  • amino acids 43 to 489 (447 residues), 175.7 bits, E=2.6e-55

Best Hits

KEGG orthology group: K00200, formylmethanofuran dehydrogenase subunit A [EC: 1.2.99.5] (inferred from 96% identity to vap:Vapar_3066)

Predicted SEED Role

"Formylmethanofuran dehydrogenase subunit A (EC 1.2.99.5)" in subsystem Methanogenesis (EC 1.2.99.5)

MetaCyc Pathways

• methyl-coenzyme M oxidation to CO₂ II (4/6 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (12/18 steps found)
• methanogenesis from H₂ and CO₂ (3/6 steps found)
• methyl-coenzyme M oxidation to CO₂ I (3/6 steps found)
• reductive acetyl coenzyme A pathway II (autotrophic methanogens) (3/6 steps found)
• methoxylated aromatic compound degradation II (4/9 steps found)
• superpathway of methanogenesis (5/21 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (24/56 steps found)

KEGG Metabolic Maps

• Folate biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.2.99.5

Use Curated BLAST to search for 1.2.99.5

Protein Sequence (561 amino acids)

>ABID97_RS12655 formylmethanofuran dehydrogenase subunit A (Variovorax sp. OAS795)
MTATLGLRGGRVIDPANGCDEVRDLYLRDGRMVALAPHERATEEIDMSGCIVMAGGIDMH
THIGGGKVNLARMLMPEDHRANGNPLALPDNLLELASCGTCTPGTLATGYRYVEMGYTAA
FEPAMMACNARHTHMEMGDTPILDHGAYVMLGNDELFLRMLAERWDFERIRDYAGWTLNA
SKAMGVKVVNPGGISAFKFNQRKLDVDENHVHWQVTPRQVVHTLARALRELGVPHPLHIH
GSNLGVAGNIQSTLDTIAALDGLPGHLTHIQFHAYGTEGPKKFSSAALQLAEVVNANKNV
SIDVGQIMFGQTVTASGDTMRQHAQSGLADPRKWIGADIECEAGCGVVPFRYREQSYVNA
LQWVIGLEIFLLVDDPWRVVLTTDHPNGGPFTSYPHLIRLLMDRSFREEQLAKLHPEVAA
QAALRSITRELSLYEIAIMTRAGPARLLGLRDRGHLGEGAAADIAVYRENPDREAMFATP
EYVFKDGVLVSRAGRITAVPVGGTHFVAPDYDRGIEKRLRRHLAAEGSVNFDHIAIGHDE
LCQCCNGGRLLPAACFQDASP