Protein Info for ABID97_RS12580 in Variovorax sp. OAS795

Annotation: methenyltetrahydromethanopterin cyclohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02289: MCH
  • amino acids 11 to 326 (316 residues), 401.3 bits, E=1.5e-124
• TIGR03120: methenyltetrahydromethanopterin cyclohydrolase
  • amino acids 11 to 326 (316 residues), 367.7 bits, E=2.3e-114

Best Hits

Swiss-Prot: 56% identical to MCH_METFK: Methenyltetrahydromethanopterin cyclohydrolase (mch) from Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

KEGG orthology group: K01499, methenyltetrahydromethanopterin cyclohydrolase [EC: 3.5.4.27] (inferred from 92% identity to vap:Vapar_3082)

MetaCyc: 56% identical to methenyltetrahydropterin cyclohydrolase subunit (Methylorubrum extorquens AM1)
Methenyltetrahydromethanopterin cyclohydrolase. [EC: 3.5.4.27]

Predicted SEED Role

"N(5),N(10)-methenyltetrahydromethanopterin cyclohydrolase (EC 3.5.4.27)" in subsystem Methanogenesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 3.5.4.27)

MetaCyc Pathways

• formaldehyde oxidation VI (H₄MPT pathway) (3/4 steps found)
• methyl-coenzyme M oxidation to CO₂ II (4/6 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (12/18 steps found)
• methanogenesis from H₂ and CO₂ (3/6 steps found)
• methyl-coenzyme M oxidation to CO₂ I (3/6 steps found)
• reductive acetyl coenzyme A pathway II (autotrophic methanogens) (3/6 steps found)
• superpathway of C1 compounds oxidation to CO₂ (7/12 steps found)
• methoxylated aromatic compound degradation II (4/9 steps found)
• superpathway of methanogenesis (5/21 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (24/56 steps found)

KEGG Metabolic Maps

• Folate biosynthesis
• Methane metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.27

Protein Sequence (332 amino acids)

>ABID97_RS12580 methenyltetrahydromethanopterin cyclohydrolase (Variovorax sp. OAS795)
MSNTTLPAALSVNLMARPLVERLLADADALGLQVRRDDTGVHIVDAGIEAPGSVAAGLRV
GEICMGGLGHVNLRSGAGTEGWPTWLDVRSSQPVLACLGSQYAGWSLAATKEETGGKKFF
SLGSGPARALAVKEKLFAELGYRDHAPCGVLVLEVDRAPPKIVIDKLLRDCGLAPEALTL
ILTPTTSLAGTTQVVARVLEVALHKAHELGFALADIVEGAGTAPLPSPSADGVEAMGRTN
DAILYGGRVHLAVRGDDAAARELARALPSRNSRDHGRSFALIFKEVDYDFYKIDGALFAP
AEVWVSNIDSGNTWHGGATEMGLLQRLWLQES