Protein Info for ABID97_RS12465 in Variovorax sp. OAS795

Annotation: dicarboxylate/amino acid:cation symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 447 transmembrane" amino acids 18 to 37 (20 residues), see Phobius details amino acids 52 to 73 (22 residues), see Phobius details amino acids 85 to 107 (23 residues), see Phobius details amino acids 154 to 173 (20 residues), see Phobius details amino acids 193 to 215 (23 residues), see Phobius details amino acids 234 to 254 (21 residues), see Phobius details amino acids 316 to 355 (40 residues), see Phobius details amino acids 359 to 384 (26 residues), see Phobius details PF00375: SDF" amino acids 18 to 410 (393 residues), 381.2 bits, see alignment E=2.9e-118

Best Hits

Swiss-Prot: 85% identical to DCTA1_POLNA: C4-dicarboxylate transport protein 1 (dctA1) from Polaromonas naphthalenivorans (strain CJ2)

KEGG orthology group: K11103, aerobic C4-dicarboxylate transport protein (inferred from 95% identity to vap:Vapar_2032)

MetaCyc: 73% identical to C4 dicarboxylate/orotate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-121; TRANS-RXN-121A; TRANS-RXN-121C; TRANS-RXN-122A; TRANS-RXN0-451; TRANS-RXN0-517; TRANS-RXN0-553

Predicted SEED Role

"Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (447 amino acids)

>ABID97_RS12465 dicarboxylate/amino acid:cation symporter (Variovorax sp. OAS795)
MADTTTTPPRKLPLYRSLYVQVITAVVIGVLLGHFYPDIGAAMKPLGDGFIKLIKMIIAP
IIFCTVVVGIAGMEDMKKVGKTGGLALLYFEIVSSVALVVGLLLVNLLQPGAGMNIDPAT
LDTKAIASYTGPGKMTGTVDFLLNVIPNTVVDAFAKGEILQVLLIAVLFGFALHRFGGRG
TLVFDVIEKGSHVLFVIVGYIMKLAPIGAFGAMAFTIGKYGLGSLFSLGKLMGTFYLTCL
LFIFVVLGGIARFHGFSIWKFIKYIKEELLIVLGTSSSESVLPRMMEKMENLGANKACVG
LVIPTGYSFNLDGTSIYLTMAAVFIAQATNTPMTLMQEITLLAVLLLTSKGAAGVTGSGF
IVLAATLSAVGHVPVAGLALILGIDRFMSEARALTNLIGNGVATIVVAKWTNELDTARLQ
AGLNNENWVESQEPEVLEGARVSKMAD