Protein Info for ABID97_RS12090 in Variovorax sp. OAS795

Annotation: alpha-amylase family glycosyl hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 536 PF00128: Alpha-amylase" amino acids 29 to 375 (347 residues), 340.6 bits, see alignment E=1.3e-105 PF11941: DUF3459" amino acids 444 to 532 (89 residues), 42 bits, see alignment E=1e-14

Best Hits

KEGG orthology group: K01187, alpha-glucosidase [EC: 3.2.1.20] (inferred from 89% identity to vap:Vapar_1969)

Predicted SEED Role

"Maltodextrin glucosidase (EC 3.2.1.20)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.20

Use Curated BLAST to search for 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (536 amino acids)

>ABID97_RS12090 alpha-amylase family glycosyl hydrolase (Variovorax sp. OAS795)
MSADAWWKNGIVYQIYPRSFQDSDGDGIGDLRGIQRRLDYLVELGVDAVWISPIYPSPMA
DFGYDISDYCNIDPRFGTLDDFDALAAECKARGLKLVLDFVPNHTSDRHPWFAQSRASRG
SPKRDWYLWRDPAQGGGPPNNWLSNFGGPAWTLDEASGQYYCHSFLREQPDLNWRNPEVR
RAMYEALRFWLRRGVDGFRVDVLYHLVKDEDFRDNPDNPSWQPGQDPSHRLLPLYTTDRP
EMQDIVAEMRRVVDEFSDEASSRVLIGELYLPLERLMAYYGRTADGLLQGVQLPFNFQLI
AAPWHAVEIDRIVRNYEAALPHGAAPNWVLGNHDKPRIASRVGQERARLAAMLLLTLRGT
PTLYYGDEIGLTDVPIPPAEVQDPFEKNEPGKGLGRDPQRTPMQWSRAHAAGFTDGTPWL
RLGGDWGMRNVEAQQGDPDSMLALYKKLIALRRREPALHEGSHEQLDAGADVLAYARCAG
TRRLVVLLNFASAETAVAAHLLPPDAVVLASTHAGRTGTAKGELLLEPLEGVIVAQ