Protein Info for ABID97_RS11770 in Variovorax sp. OAS795

Annotation: ribulose-bisphosphate carboxylase large subunit family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02788: RuBisCO_large_N
  • amino acids 18 to 130 (113 residues), 27.9 bits, E=2.4e-10
• PF00016: RuBisCO_large
  • amino acids 141 to 420 (280 residues), 214.9 bits, E=1.6e-67

Best Hits

Swiss-Prot: 49% identical to OIAT_XANP2: 3-oxo-isoapionate-4-phosphate transcarboxylase/hydrolase (oiaT) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K01601, ribulose-bisphosphate carboxylase large chain [EC: 4.1.1.39] (inferred from 95% identity to vap:Vapar_1945)

MetaCyc: 49% identical to 3-oxoisoapionate-4-phosphate transcarboxylase/hydrolase (Xanthobacter autotrophicus Py2)
RXN-20936 [EC: 3.7.1.28]

Predicted SEED Role

"similar to ribulose-1,5-bisphosphate carboxylase, Type III"

MetaCyc Pathways

• Rubisco shunt (10/10 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (21/26 steps found)
• Calvin-Benson-Bassham cycle (11/13 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
• oxygenic photosynthesis (12/17 steps found)
• D-apionate degradation III (RLP transcarboxylase/hydrolase) (1/3 steps found)
• nucleoside and nucleotide degradation (archaea) (1/10 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.39

Use Curated BLAST to search for 3.7.1.28 or 4.1.1.39

Protein Sequence (423 amino acids)

>ABID97_RS11770 ribulose-bisphosphate carboxylase large subunit family protein (Variovorax sp. OAS795)
MAPAERIHARYLIETPLEPAAVAEVMAGEQSCGTFTRVEGETDALRERARATVEAITELA
PAEAPALPNALLERKGTRGPWRRAHVDISFPVANIGANLPTLAATVSGNLYDLGEVTGLR
LESLRLPAAYRARFEMPRAGVAGTRRTTGVASGALVGTIIKPNVGLSAAETAGLVARLCA
AGVDFIKDDEVCADPAHAPLAERVPAVMDVVRAHQQRTGKHVMVAFNITDETDAMKRHAD
LVEREGGSCVMASLNWCGHAGIQTLRRHTGLALHGHRNGYGALSRHPLLGISFQAYQTLW
RLAGVDHMHVHGLQGKFSQPDAEVIESARDCFTPLTDAADDRVMPAFSSGQWAGTVPATW
AAIGSDDLLFMAGGGILAHPDGAAAGVASIRQAWSAARAGTALDEAARSAPELARALSFF
GKP