Protein Info for ABID97_RS10915 in Variovorax sp. OAS795

Annotation: 4-hydroxy-tetrahydrodipicolinate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 36% identical to DAPA2_CLOAB: 4-hydroxy-tetrahydrodipicolinate synthase 2 (dapA2) from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)

KEGG orthology group: K01714, dihydrodipicolinate synthase [EC: 4.2.1.52] (inferred from 86% identity to vap:Vapar_1836)

Predicted SEED Role

"4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7)" (EC 4.3.3.7)

MetaCyc Pathways

aspartate superpathway (24/25 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
L-lysine biosynthesis I (9/9 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
L-lysine biosynthesis III (6/7 steps found)
L-lysine biosynthesis VI (6/7 steps found)
dipicolinate biosynthesis (3/4 steps found)
L-lysine biosynthesis II (6/9 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.52, 4.3.3.7

Use Curated BLAST to search for 4.2.1.52 or 4.3.3.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (298 amino acids)

>ABID97_RS10915 4-hydroxy-tetrahydrodipicolinate synthase (Variovorax sp. OAS795)
MPQSSSLSASPDFSGLWIPLVTPFRGGAIDHAALSALTRRLAGDGVAGFVVCGSTGEAAA
LDETEQLAALETVQAAAEGLPIVMGLSGYHLGKTTAWVRQLAEHRLAGLLVPAPHYIRPS
QAGLLEWFRAIADASPVPVLIYDIPYRTGATLARETLLALAEHPRIRGVKDCGGDMAKTR
ALIADGRLQVLSGEDHQIFATVAEGGAGAIAASGHVQTRRFVQVLRLLAENRLAQARAEW
QPLRPLIELLFAEPNPGPVKALLAHGGSMSGELRSPMTRATDALRDRLVALDAGLSRR