Protein Info for ABID97_RS09545 in Variovorax sp. OAS795

Annotation: HAD-IA family hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 237 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00702: Hydrolase" amino acids 22 to 198 (177 residues), 98 bits, see alignment E=1.9e-31 PF12710: HAD" amino acids 24 to 191 (168 residues), 30.3 bits, see alignment E=1.1e-10 TIGR01449: phosphoglycolate phosphatase, bacterial" amino acids 24 to 216 (193 residues), 170.2 bits, see alignment E=6.9e-54 PF13419: HAD_2" amino acids 24 to 203 (180 residues), 110 bits, see alignment E=3.1e-35 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 108 to 202 (95 residues), 33.8 bits, see alignment E=5.4e-12 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 119 to 198 (80 residues), 38.8 bits, see alignment E=1.8e-13 PF13242: Hydrolase_like" amino acids 160 to 212 (53 residues), 43.8 bits, see alignment E=3.9e-15

Best Hits

Swiss-Prot: 40% identical to MUPP_PSEAE: N-acetylmuramic acid 6-phosphate phosphatase (mupP) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01091, phosphoglycolate phosphatase [EC: 3.1.3.18] (inferred from 90% identity to vap:Vapar_1623)

MetaCyc: 38% identical to N-acetyl-beta-muramate 6-phosphate phosphatase (Pseudomonas putida KT2440)
RXN-18659 [EC: 3.1.3.105]

Predicted SEED Role

"Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase" in subsystem 2-phosphoglycolate salvage

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.18

Use Curated BLAST to search for 3.1.3.105 or 3.1.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (237 amino acids)

>ABID97_RS09545 HAD-IA family hydrolase (Variovorax sp. OAS795)
MFATQKPALAAGSPAVTAAKVQAVLFDLDGTLIDSAPDLGAAADKMRTDRGLESFPLERY
RFMAGAGARGMLGVAFGITPDAPEFPALREEFFVAYENRMLLNTQVFDGVQALIDAICAR
GLAWGVVTNKSARFTDPLTRAIALFDSAGAIVSGDTTPFSKPHPGPLHEAARRLGVPSSA
CIYVGDDERDIIAGRAAGMRTVAATYGYMGAQADATLWEADAAITSPLELLQYLNRA