Protein Info for ABID97_RS06630 in Variovorax sp. OAS795

Annotation: bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 13 to 473 (461 residues), 580.9 bits, E=9.2e-179
• PF01128: IspD
  • amino acids 13 to 134 (122 residues), 32 bits, E=2.8e-11
• PF12804: NTP_transf_3
  • amino acids 14 to 142 (129 residues), 76.4 bits, E=7.5e-25
• PF00483: NTP_transferase
  • amino acids 15 to 223 (209 residues), 49.6 bits, E=1e-16
• PF14602: Hexapep_2
  • amino acids 413 to 446 (34 residues), 19 bits, see alignment (E = 2.4e-07)
• PF00132: Hexapep
  • amino acids 413 to 447 (35 residues), 28.7 bits, 1.9e-10

Best Hits

Swiss-Prot: 75% identical to GLMU_POLSJ: Bifunctional protein GlmU (glmU) from Polaromonas sp. (strain JS666 / ATCC BAA-500)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 93% identity to vap:Vapar_0999)

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• peptidoglycan recycling I (10/14 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• chitin biosynthesis (5/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (10/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (476 amino acids)

>ABID97_RS06630 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU (Variovorax sp. OAS795)
MNQIPQDNQGPVDVVIMAAGKGTRMKSRLPKVLHRLAGRALLAHVTDTAARIGARHVVVV
TGHGADEVEAAMSASAVGATLQFARQEPQLGTGHAVQQAVPLLPDDSTVLVLSGDVPLIG
EDTLRALIAASAGQRLALLTIEFGDPTGYGRIVRAEGEGAGDVTAIVEQKDATEAQRQIR
EIYSGVMAVPAGLLKGWLARLDNRNAQGEYYLTDIVKLAAADGVPVVAHVIADALQVAGI
NSPAQLASLERAWQLRQADALMAQGVRLADPARFDLRGTLACEADVEIDVNCVFEGAVYL
GEGVRIGANCVIANARIEAGAVIHPFTHIDGEKAGVTVGERSLVGPFARLRPGAQLGTEV
HIGNFVEVKNSTLADGAKANHLAYLGDASVGRRVNYGAGSITANYDGANKHRTVIEDDVH
VGSNCVLVAPVTIGAGGTIGGGSTVNKSTEPGALTVARSKPVSFSNWKRPQKKPKA