Protein Info for ABID97_RS06240 in Variovorax sp. OAS795

Annotation: UDP-N-acetylmuramate--L-alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 7 to 105 (99 residues), 100.8 bits, E=6.7e-33
• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 7 to 458 (452 residues), 538 bits, E=1.1e-165
• PF08245: Mur_ligase_M
  • amino acids 110 to 293 (184 residues), 91 bits, E=1.5e-29
• PF02875: Mur_ligase_C
  • amino acids 314 to 404 (91 residues), 51.4 bits, E=1.7e-17

Best Hits

Swiss-Prot: 98% identical to MURC_VARPS: UDP-N-acetylmuramate--L-alanine ligase (murC) from Variovorax paradoxus (strain S110)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 98% identity to vap:Vapar_0920)

MetaCyc: 53% identical to UDP-N-acetylmuramate--L-alanine ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramate--L-alanine ligase. [EC: 6.3.2.8]

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Protein Sequence (476 amino acids)

>ABID97_RS06240 UDP-N-acetylmuramate--L-alanine ligase (Variovorax sp. OAS795)
MKHAIRHIHFVGIGGSGMSGIAEVLFNLGYRITGSDLADSATLRRLAGLGIGTFVGHAAA
HIDGADAVVTSTAVQSDNPEVLAAREKRIPVVPRALMLAELMRLKQGIAIAGTHGKTTTT
SLVASVLDAAGLDPTFVIGGRLNSAGANAQLGSGDYIVVEADESDASFLNLLPVMAVVTN
IDADHMETYGHDFEKLKKAFVDFLHRMPFYGVAILCTDDPAVRDIVSHVTCPVTSYGFGE
DAQVRAVDVRAVGGQMHFTAQRRNGVTLPDLPIVLNLPGEHNVRNALSVIAVAVELGIPD
EAVQRGLAGFKGVGRRFQSYGEVAVQGPQAGSFTVIDDYGHHPVEMAATIAAARGAFPGR
RLVLAFQPHRYTRTRDCFEDFVKVIGNADAVLLGEVYAAGEPPIVAANGRTLARALRVAG
KVEPVFVDDIGAMPQAILDNARDGDVVLCMGAGSIGAVPGKVVEIAGAARGAEVNP