Protein Info for ABID97_RS04665 in Variovorax sp. OAS795
Annotation: acyl-CoA dehydrogenase family protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 54% identical to ACADL_MOUSE: Long-chain specific acyl-CoA dehydrogenase, mitochondrial (Acadl) from Mus musculus
KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 95% identity to vap:Vapar_0443)MetaCyc: 53% identical to long-chain-acyl-CoA dehydrogenase monomer (Homo sapiens)
LONG-CHAIN-ACYL-COA-DEHYDROGENASE-RXN [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; RXN-21775 [EC: 1.3.8.8, 1.3.8.7]
Predicted SEED Role
"Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)" in subsystem Isoleucine degradation (EC 1.3.99.2)
MetaCyc Pathways
- oleate β-oxidation (29/35 steps found)
- adipate degradation (5/5 steps found)
- fatty acid salvage (5/6 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
- 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
- pyruvate fermentation to butanol II (engineered) (4/6 steps found)
- pyruvate fermentation to hexanol (engineered) (7/11 steps found)
- (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
KEGG Metabolic Maps
- Butanoate metabolism
- Fatty acid metabolism
- Propanoate metabolism
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.2, 1.3.99.3
Use Curated BLAST to search for 1.3.8.7 or 1.3.8.8 or 1.3.99.2 or 1.3.99.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (387 amino acids)
>ABID97_RS04665 acyl-CoA dehydrogenase family protein (Variovorax sp. OAS795) MIERTLFGADHEAFRDSFRRFMDKEIAPFHAAWEDQGYVDRAVWSKAGENGFLCMALPEE HGGAGADLLYSVIQFEELWARGFTGIGFGLHSEIVAPYILRYGTGAQKKRYLPRLASGEM VGAIAMSEPGAGSDLQAVKASAIRQADGSYLLNGSKTFITNGWHADLVIVVAKTDPAAGA KGTSLFLVERGMAGFEKGKRLKKLGLKAQDTSELFFNDVRLPADALLGDAAQLNRGFVCL MEQLPWERLQIAISAVAASQSAIDQTVAYVKDRKVFGQPVANYQNTRYTLAELQTEVQVA QVFVDKCIELLMAEKLDTATASMAKYWTTDLQCKVMDECVQLHGGYGFMWEYPITRAYAD ARVQRIYGGTNEIMKEVITRAMGLGGR