Protein Info for ABID97_RS04600 in Variovorax sp. OAS795

Annotation: glutamate-5-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 17 to 285 (269 residues), 47.2 bits, E=6.5e-17
  • amino acids 322 to 392 (71 residues), 26.3 bits, E=1.4e-10
• TIGR00407: glutamate-5-semialdehyde dehydrogenase
  • amino acids 17 to 419 (403 residues), 473.2 bits, E=3.1e-146

Best Hits

Swiss-Prot: 79% identical to PROA_POLNA: Gamma-glutamyl phosphate reductase (proA) from Polaromonas naphthalenivorans (strain CJ2)

KEGG orthology group: K00147, glutamate-5-semialdehyde dehydrogenase [EC: 1.2.1.41] (inferred from 97% identity to vap:Vapar_0425)

Predicted SEED Role

"Gamma-glutamyl phosphate reductase (EC 1.2.1.41)" in subsystem Proline Synthesis (EC 1.2.1.41)

MetaCyc Pathways

• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-ornithine biosynthesis II (2/3 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.41

Protein Sequence (430 amino acids)

>ABID97_RS04600 glutamate-5-semialdehyde dehydrogenase (Variovorax sp. OAS795)
MNAFNVSEHMQALGLQAKAAAFLMARADAATKNRALKALARRLREAGPGLAESNEKDLAR
ATAAGLPAPMVDRLKLTPKVIETVALGCEQLAGMADVIGEIIGMKQQPSGIRVGQMRVPI
GVFGMIYESRPNVTIEAASLAIKSGNAAILRGGSEAIESNKALALLVSAALAEAGLPVEA
VQLVQTTDREAVGQLIAMPEFVDVIIPRGGKGLIERISRDAKVPVIKHLDGNCHVYVDDT
AEFEMALRIVDNAKTQKYSPCNAAEGLLVSAVVAEDFLPRIAAIFAAKGVEMRCDPVAAR
ILRADESLAPGARIVDAVESDWSEEYLAAVISIKVVAGVDEAIAHINRYSSHHTDAIVTR
DHVHAQRFLREVDSASVMVNASTRFADGFEFGLGAEIGISTDKFHARGPVGIEGLTSLKY
VVLGQGEVRS