Protein Info for ABID97_RS02825 in Variovorax sp. OAS795

Annotation: heavy metal translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 762 transmembrane" amino acids 96 to 115 (20 residues), see Phobius details amino acids 125 to 142 (18 residues), see Phobius details amino acids 162 to 180 (19 residues), see Phobius details amino acids 195 to 214 (20 residues), see Phobius details amino acids 348 to 370 (23 residues), see Phobius details amino acids 379 to 404 (26 residues), see Phobius details amino acids 702 to 720 (19 residues), see Phobius details amino acids 726 to 744 (19 residues), see Phobius details PF00403: HMA" amino acids 17 to 77 (61 residues), 59.6 bits, see alignment 6.5e-20 TIGR01511: copper-translocating P-type ATPase" amino acids 142 to 745 (604 residues), 553.3 bits, see alignment E=1.4e-169 TIGR01525: heavy metal translocating P-type ATPase" amino acids 160 to 744 (585 residues), 604.1 bits, see alignment E=6.2e-185 TIGR01494: HAD ATPase, P-type, family IC" amino acids 204 to 451 (248 residues), 155.7 bits, see alignment E=2.9e-49 amino acids 559 to 725 (167 residues), 124.1 bits, see alignment E=1.1e-39 PF00122: E1-E2_ATPase" amino acids 232 to 332 (101 residues), 100.4 bits, see alignment E=8.8e-33 PF00702: Hydrolase" amino acids 427 to 653 (227 residues), 115.5 bits, see alignment E=8.2e-37 PF12710: HAD" amino acids 552 to 649 (98 residues), 30.7 bits, see alignment E=8.2e-11

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 89% identity to vap:Vapar_0031)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (762 amino acids)

>ABID97_RS02825 heavy metal translocating P-type ATPase (Variovorax sp. OAS795)
MDNLLHSPLLPMSTLDLSVGGMTCASCVMRVERALKSVPGVQDVSVNLATESARVVVADG
EDLDARLRRAVRAAGYEPRTASGPIGEAAALSPWHGFGPVAVGLLLSIPLLAPMLGQPFG
QDWMLAPWIQLLLAAPVQFWLGARFYRAGWHAARAGTGNMDLLVALGTSAAFGLSLWLWW
RAATEGHAAHGPVPHLYFEASAVVITLVLLGKWLEARAKRQATSAIRALQQLRPELAHLV
GTRGESDVPIAEVMVGDLLAVRPGERVPADARVVEGESEVDESMLTGEPLPVPRKPGDAL
TGGAVNGDGRMVIEVKAVGAESVLSRIIRLVEDAQAAKAPIQRLVDRVAAVFVPVVLVIA
LATLAGWLLAGAGIERAMIHAVAVLVIACPCALGLATPVAVMAGTGVAARRGILVKDARA
LELAHRVDTVAFDKTGTLTLGRPVLTALVPLVPLTGGMHEAGLLATAASLQGGSEHPLAR
AVTAAAAQRGVAAPPLGAMQALPGRGVRGEVNGEQWAIASLRWCAELGVVPDATQVERLH
SLGATVSALLRFDGAGGAQAQALLAFADEPKPEAAEAVRALRGRGLRVVMISGDNRRAAE
AMAARLGIAAADVRADVLPADKAAQVGALKQGGHVVAMVGDGANDAPALAAADVGIAMAP
SGGGTDVAMEAAGITLMRGDLALVAEAFELSARTVAKIRQNLFWAFAYNVAGIPLAAFGL
LSPVVAGAAMALSSVSVMANALLLRRWKPSGPSAQSISSASR