Protein Info for ABID97_RS00505 in Variovorax sp. OAS795

Annotation: lipoyl synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF16881: LIAS_N
  • amino acids 32 to 79 (48 residues), 30.7 bits, 3.6e-11
• TIGR00510: lipoyl synthase
  • amino acids 38 to 320 (283 residues), 422.8 bits, E=3.7e-131
• PF04055: Radical_SAM
  • amino acids 94 to 257 (164 residues), 89.7 bits, E=2.6e-29

Best Hits

Swiss-Prot: 98% identical to LIPA_VARPS: Lipoyl synthase (lipA) from Variovorax paradoxus (strain S110)

KEGG orthology group: K03644, lipoic acid synthetase [EC: 2.8.1.8] (inferred from 98% identity to vap:Vapar_4881)

MetaCyc: 64% identical to lipoyl synthase (Escherichia coli K-12 substr. MG1655)
Lipoyl synthase. [EC: 2.8.1.8]

Predicted SEED Role

"Lipoate synthase" in subsystem Lipoic acid metabolism

MetaCyc Pathways

• lipoate biosynthesis and incorporation I (2/2 steps found)
• lipoate biosynthesis and incorporation III (Bacillus) (2/3 steps found)
• lipoate biosynthesis and incorporation V (mammals) (2/3 steps found)
• lipoate biosynthesis and incorporation IV (yeast) (4/7 steps found)
• lipoate biosynthesis and incorporation II (1/3 steps found)

KEGG Metabolic Maps

• Lipoic acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.8

Protein Sequence (326 amino acids)

>ABID97_RS00505 lipoyl synthase (Variovorax sp. OAS795)
MSSTEVVRDAQSAENYNPLAKQKAAAKLSRIPVKVVQGEVLKKPEWIRVKAGSPTTRFYE
IKQILRESNLHTVCEEASCPNIGECFGNGTATFMIMGDKCTRRCPFCDVGHGRPDPLDKD
EPLNLAKTIAKLRLKYVVITSVDRDDLRDGGSQHFVDCITHIRELSPMTQIEILVPDFRG
RDDRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQFSLNLLKKFKALHPAVPTKSGI
MVGLGETDEEILQVMRDMRAHGIDMLTIGQYLSPSGSHLPVRRYVHPDTFKMFEEEAYKM
GFSHAAVGAMVRSSYHADQQAHAAGV