Protein Info for ABI39_RS15270 in Phocaeicola dorei CL03T12C01

Annotation: type I pullulanase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 664 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details TIGR02104: pullulanase, type I" amino acids 36 to 641 (606 residues), 840.5 bits, see alignment E=3e-257 PF02922: CBM_48" amino acids 43 to 108 (66 residues), 42.3 bits, see alignment E=1.1e-14 PF00128: Alpha-amylase" amino acids 198 to 291 (94 residues), 39.7 bits, see alignment E=7.1e-14 amino acids 323 to 524 (202 residues), 25.9 bits, see alignment E=1.1e-09 PF21653: pulA_all-beta" amino acids 579 to 638 (60 residues), 67.3 bits, see alignment 1.1e-22

Best Hits

KEGG orthology group: K01200, pullulanase [EC: 3.2.1.41] (inferred from 99% identity to bvu:BVU_3034)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-) / Pullulanase (EC 3.2.1.41)" in subsystem Glycogen metabolism or Trehalose Biosynthesis or Maltose and Maltodextrin Utilization (EC 3.2.1.-, EC 3.2.1.41)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 3.2.1.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (664 amino acids)

>ABI39_RS15270 type I pullulanase (Phocaeicola dorei CL03T12C01)
MNVRHVIWASIVSTTISCQSVKKEYNSFDEYPVREDALTEMEYSPAETKFSLWAPTAEEV
RVLLFESGNEGSASNTFPMEMGENGTWTISIKEDLKGKFYTFNVKVNGKWLGDTPGIMAK
AVGVNGKRAAVLDLRSTDPEGWENDVRPPLKNYADIMVYEMHHRDFSLDSVSGIQNKGKF
LALTEQGTISSSGEKTGIDHLKELGITHVHLLPSYDYASVDETKLDKAQYNWGYDPQNYN
VPDGSYSTDPYKPDVRIREFKQMVQALHKAGIRVVLDVVYNHTFNTDESNFERTVPGYFY
RQTKDGQWANGSGCGNETASDRAMMRKYMIESILYWINEYHIDGFRFDLMGIHDIETMNE
IRAAIDKIDPSIFMYGEGWAASSPQLEADKLAMKANVEKMPRIAAFSDEMRDGLRGGWDD
DTKGAFLVGEPGHEMSIKFGVVGAIEHPQVISDSVNYSKKPWALQPTQMISYVSCHDDMC
LADRLKATMPDASVEELAALQKLAETFVFTSQGVPFIFAGDEMMRDKKGVHNSYNSPDSI
NTIDWKNKTVHKDVFEYVKGLIAMRKAHPAFRMGDADMVRRQLEFLPVKNTNVVAFILKD
NANGDSWKNIIVALNSRAEPVKLDIPSGKYTVICKDGKINMKGLGQVSGAELMVPARSAM
IIHQ