Protein Info for ABI39_RS03545 in Phocaeicola dorei CL03T12C01

Annotation: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 7 to 425 (419 residues), 601.9 bits, E=2.9e-185
• PF01053: Cys_Met_Meta_PP
  • amino acids 8 to 425 (418 residues), 431.2 bits, E=2.7e-133
• PF00155: Aminotran_1_2
  • amino acids 69 to 187 (119 residues), 23.9 bits, E=2.3e-09

Best Hits

Swiss-Prot: 46% identical to CYSD_EMENI: Homocysteine synthase (cysD) from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 99% identity to bvu:BVU_0770)

MetaCyc: 45% identical to O-acetylhomoserine sulfhydrylase (Leptospira meyeri)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
• aspartate superpathway (19/25 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-homocysteine biosynthesis (1/2 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (8/12 steps found)
• L-methionine biosynthesis III (2/4 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (12/18 steps found)
• L-methionine biosynthesis II (3/6 steps found)
• homocysteine and cysteine interconversion (1/4 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (2/6 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (1/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (1/5 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (4/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Protein Sequence (425 amino acids)

>ABI39_RS03545 O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (Phocaeicola dorei CL03T12C01)
MDKKYSRETLCVQAGWTPKKGEPRVLPIYQSTTFKYDTSEQMARLFDLEDSGYFYTRLQN
PTNDAVAAKIAALEGGVGAMLTSSGQAANFYAIFNICQAGDHFVCSSTIYGGTFNLFGVT
LKKLGIECTFIDANASEEEIDQAFRPNTKALFGETISNPSIDVLDIEKFARIAHKHGVPL
IVDNTFATPINCRPFEWGADIVTHSTTKYMDGHATSVGGAIVDSGNFDWEAHADKFPGLT
QPDESYHGLTYTKAFGKMAYMTKATAQLMRDLGSIQSPQNSFLLNLGLETLHLRVPRHCE
NAQKVAEWLEANPKVKWVNYCGLKSSKYYDLAQKYMPNGSCGVIAFGLNGTREEAIEFMD
RLKFICIVTHVADARTCVLHPASHTHRQLTDEQLREAGVAPDLIRLSVGIENVNDIIADI
EQALQ