Protein Info for ABI39_RS01405 in Phocaeicola dorei CL03T12C01

Annotation: tRNA (guanosine(46)-N7)-methyltransferase TrmB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 252 PF13847: Methyltransf_31" amino acids 45 to 108 (64 residues), 28.1 bits, see alignment E=2.4e-10 PF02390: Methyltransf_4" amino acids 45 to 212 (168 residues), 92.2 bits, see alignment E=4.2e-30

Best Hits

Swiss-Prot: 84% identical to TRMB_BACFR: tRNA (guanine-N(7)-)-methyltransferase (trmB) from Bacteroides fragilis (strain YCH46)

KEGG orthology group: K03439, tRNA (guanine-N7-)-methyltransferase [EC: 2.1.1.33] (inferred from 98% identity to bvu:BVU_0267)

Predicted SEED Role

"tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33)" (EC 2.1.1.33)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (252 amino acids)

>ABI39_RS01405 tRNA (guanosine(46)-N7)-methyltransferase TrmB (Phocaeicola dorei CL03T12C01)
MGKGKLEKFADMREYPHVFEYPYSVADNVTFDMKGNWNRDFFKNDNPIVLELGCGRGEYT
VGLGGMYSDKNFIGVDIKGARMWTGATEALKNGMKNVAFLRTNIEIIDRFFAPSEVSEIW
LTFSDPQMKKVTKRLTSTYFMERYRKFLKPDGLIHLKTDSNFMFTYTRYMVEENKLPVEF
MTEDLYHSGLVDDILGIRTYYEQQWLDRGLNIKYMKFRLPHEKELHEPDVEIELDEYRSY
NRSKRSGLKTSK