Protein Info for A4249_RS14940 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: NAD(+) synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K01950, NAD+ synthase (glutamine-hydrolysing) [EC: 6.3.5.1] (inferred from 80% identity to bsb:Bresu_0326)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

aspartate superpathway (21/25 steps found)
NAD de novo biosynthesis I (6/6 steps found)
NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
NAD salvage pathway I (PNC VI cycle) (6/7 steps found)
NAD de novo biosynthesis III (5/6 steps found)
NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)
NAD salvage pathway II (PNC IV cycle) (4/5 steps found)
NAD salvage pathway V (PNC V cycle) (4/5 steps found)
NAD de novo biosynthesis II (from tryptophan) (5/9 steps found)
NAD salvage (plants) (6/11 steps found)
superpathway of NAD biosynthesis in eukaryotes (7/14 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.5 or 6.3.5.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161IJP1 at UniProt or InterPro

Protein Sequence (679 amino acids)

>A4249_RS14940 NAD(+) synthase (Brevundimonas sp. GW460-12-10-14-LB2)
MADALFHSPKTHGFVRVAAATPVSHPGDPRANGQEHVALIRQAGEQGVDLMVFPELSLSA
YAIDDLHMQAALLDEVERQIAVVAAATAEADVVAVVGAPIRNGDALYNCAVVIGSGEVLG
VVPKTYLPNYREYYEKRWFAPATARAEDVLRLNGETVDFASGLLFEAVNRPGFVFSVEIC
EDYWAPLPPSTRAALAGARILLNLSASNIVIGKADERAMLSASHSARTLSAYVFAASGWG
ESTTDLAWDGQATIHELGSKLAEGERFALDSHLTIADVDVDRIGLDRLRNGTFAECARNE
GEPAAVVPFMARQDHEASELIRPLDRFPFVPDDAGRLDQDCYEAFNIQVQGLMRRMTATG
AKSLVIGVSGGLDSTQALLVACRAFDRLDLPRTDILAFTMPAFATSDATKSNAWALMTAL
GVTGAEIDIRPAAEQMFKDIGHPYAEGQPVHDITFENVQAGLRTDYLFRLANQHRAFVLG
TGDLSELALGWATYGVGDHMSHYNVNGGVAKTLIRHLIRWVAAGDLIGAGARDTLHAILD
TEISPELVPAKDGVIQSTEGTVGPYALNDFFLFYISRFGMTPSKVAFLAHQAWGDAGSGH
WPANTPDDEKVEYDLASIKGWLRKFLIRFFQTAQFKRSALPNGPKVVTGGSLSPRGDWRA
PSDGNARVWLDELDANVPD