Protein Info for A4249_RS13295 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: NADP-dependent malic enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00390: malic
  • amino acids 25 to 156 (132 residues), 85.7 bits, E=5.6e-28
• PF03949: Malic_M
  • amino acids 171 to 366 (196 residues), 80.1 bits, E=2.5e-26
• PF01515: PTA_PTB
  • amino acids 435 to 751 (317 residues), 271 bits, E=2.4e-84

Best Hits

Swiss-Prot: 61% identical to MAO2_RHIME: NADP-dependent malic enzyme (tme) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00029, malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+) [EC: 1.1.1.40] (inferred from 83% identity to bsb:Bresu_0511)

Predicted SEED Role

"NADP-dependent malic enzyme (EC 1.1.1.40)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 1.1.1.40)

MetaCyc Pathways

• gluconeogenesis I (10/13 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (5/7 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (8/14 steps found)
• methylgallate degradation (2/6 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (3/8 steps found)
• superpathway of vanillin and vanillate degradation (3/10 steps found)
• syringate degradation (3/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.40

Sequence Analysis Tools

See A0A160HZI6 at UniProt or InterPro

Protein Sequence (776 amino acids)

>A4249_RS13295 NADP-dependent malic enzyme (Brevundimonas sp. GW460-12-10-14-LB2)
MPDQTEKQTFSDDEALDFHRIPTPGKISMAPTKPMATQRDLSLAYSPGVAVPVLAIAADA
DKAYDYTSKGNLVAVISNGTAILGLGNLGHMASKPVMEGKSVLFKRFADVDSFDVEVKTT
DPDEFITVVKNIGDTWGGINLEDIKSPECFVIESELQDLLDIPVFHDDQHGTAIISTAGL
INAVHIAGKKLDEIKVVLAGAGAAGLSSIALMKAAGVKAENTVICDRDGVVYKGRDHGMD
QWKAAHATDTPLRTLAEAMVGADVVLGLSAKGAITKEMVASMAPNPIIFAMANPDPEITP
EDVKSVRSDAIIATGRSDYVNQVNNVLAFPYLFRGALDVRARRVNHEMKIACAQALAALA
REDVPDEVAAAYRGRKLKFGPDYIIPTPFDPRLIWYIPPFIAQAAMDTGVARVQIEDMDA
YRHALRSRVDPSAALMQKISSAVRGGPNKRVVFAEGEEPAVIRAAWGFKQADLGTPILVG
REDLIRQNAAEAGLNFDELGIEITNARVSDHNVEDTDWLYEKLQRRGYLRRDVQRMINQD
RNYFAATLVAKGRADAMVAGVTRNFNMALKEVQRVLDVDDTLIGLSILLAKGRTLFVADT
TIHELPNAEELADIAVKAADTVRKLGRNPRVAFLSYSTFGNPPGDRAEKVREAIRILDKK
GVDFEYEGEMPPEVALDPKGHSAYAFNRLTKPANVLVMPAIHSAAISTKLVQALGGATVI
GPLLVGLEKPVQIVSLGASVSEIITAATFAAYDAGPAFQGSGLTSAPRPPAAVVER