Protein Info for A4249_RS12355 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01749: beta-hydroxyacyl-(acyl-carrier-protein) dehydratase FabA
  • amino acids 8 to 172 (165 residues), 307.1 bits, E=1.6e-96
• PF07977: FabA
  • amino acids 31 to 160 (130 residues), 179.1 bits, E=1.8e-57

Best Hits

Swiss-Prot: 83% identical to FABA_CAUVN: 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) from Caulobacter vibrioides (strain NA1000 / CB15N)

KEGG orthology group: K01716, 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase [EC: 4.2.1.60] (inferred from 88% identity to bsb:Bresu_0579)

MetaCyc: 71% identical to beta-hydroxyacyl-acyl carrier protein dehydratase/isomerase (Escherichia coli K-12 substr. MG1655)
Trans-2-decenoyl-[acyl-carrier-protein] isomerase. [EC: 5.3.3.14]; 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase. [EC: 5.3.3.14, 4.2.1.59]; 4.2.1.59 [EC: 5.3.3.14, 4.2.1.59]

Predicted SEED Role

"3-hydroxyacyl-[acyl-carrier-protein] dehydratase, FabA form (EC 4.2.1.59)" (EC 4.2.1.59)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (18/20 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
• (5Z)-dodecenoate biosynthesis I (6/6 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• (5Z)-dodecenoate biosynthesis II (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• cis-vaccenate biosynthesis (4/5 steps found)
• fatty acid elongation -- saturated (4/5 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (9/12 steps found)
• palmitate biosynthesis III (21/29 steps found)
• 8-amino-7-oxononanoate biosynthesis I (8/11 steps found)
• stearate biosynthesis IV (4/6 steps found)
• anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (3/5 steps found)
• tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
• biotin biosynthesis I (8/15 steps found)
• 2-allylmalonyl-CoA biosynthesis (2/8 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (26/205 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.59

Use Curated BLAST to search for 4.2.1.59 or 4.2.1.60 or 5.3.3.14

Sequence Analysis Tools

See A0A160I0J3 at UniProt or InterPro

Protein Sequence (177 amino acids)

>A4249_RS12355 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA (Brevundimonas sp. GW460-12-10-14-LB2)
MTQSQYPSSFDHEALLASGRGELFGPGNAQLPAPPMLMFDRITTINADGGEHGKGYVEAE
LDIHPDLWFFQCHFIGDPVMPGCLGLDAMWQLVGFYLGWIGGPGRGRALGVGEVKFTGQV
TPDIKKVVYKINLKRVINRKLVMGIADGVLEADGQVIYTCNDMRVGLFGAKDEAAGG