Protein Info for A4249_RS08445 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 932 transmembrane" amino acids 537 to 539 (3 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 3 to 912 (910 residues), 1039.8 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 15 to 611 (597 residues), 497.9 bits, see alignment E=5.5e-153 PF09334: tRNA-synt_1g" amino acids 43 to 93 (51 residues), 31.4 bits, see alignment 2.3e-11 amino acids 401 to 615 (215 residues), 34.3 bits, see alignment E=2.9e-12 PF13603: tRNA-synt_1_2" amino acids 261 to 327 (67 residues), 28 bits, see alignment 3.6e-10 PF08264: Anticodon_1" amino acids 653 to 804 (152 residues), 116.8 bits, see alignment E=1.9e-37 PF10458: Val_tRNA-synt_C" amino acids 865 to 930 (66 residues), 65.5 bits, see alignment 1.1e-21

Best Hits

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160HYX9 at UniProt or InterPro

Protein Sequence (932 amino acids)

>A4249_RS08445 valine--tRNA ligase (Brevundimonas sp. GW460-12-10-14-LB2)
MLEKTFEPQAAEPRLYAQWEESGLFAPRTDGAAEAYSIVIPPPNVTGSLHIGHALNNTLQ
DILARYHRMKGKAVLWLPGTDHAGIATQMVVERQLAAASNVGRRDMGRDAFIEKIWEWKA
ESGGTIVRQLRRLGASCDWSRERFTLDEGLNAAVRKVFVQLHKEGLIYRDKRLVNWDPHF
QTAISDLEVEQREVDGAYWHFAYPLADEETYEHPVIFDDEGNATEWETRNFIVVATTRPE
TMLGDTGVAVHPNDERYAAIVGKSVILPITGRRIPIVADEYADPTKGSGAVKITPAHDFN
DFQVGKRAGLPAINVMDAFGRILNFDAAYKGVDAEGRTTEDFEILRLVESFPDFAGLDRF
AARKAIVARAEEEGWLREIEKTKHVVPHGDRSGVVIEPWLTDQWYVDAKVLAQPALKAVE
QGDTVFEPKSYEKIYFEWLRNIEPWCISRQLWWGHRIPAWYGPNGEIYVAETEEDAREQA
MADYDSEVALTQDEDVLDTWFSSAVWPFSTMGWPEKTEDLERFYPTSDLVTAADIIFFWV
ARMMMMGQHFMGEVPFKRVIINGLVRDEKGQKMSKSKGNVIDPLGIIDELGADPLRFTMA
ILSGTRDIKLSKQRIEGYRNFGTKLWNAARFSQMNEARRIEGFDPASVGQTINRWIRGEL
TKAERAVSEAIEGGRFDDAAGALYRFVWNVFCDWYLELAKPVFQGADEAAKAETRAMTAW
TLDQTLKLLHPVMPFVTEELWAELGKEGAPRDLARDGLLIGAEWPVLPDAFIDASAEAEI
GWLVDLVGEIRGLRAEMNVPPSAKPPLAFVAPDAVTAERMARHRDLILTLGRVSEVGSAD
AAPTGAVTFVSGGSTIALSLAGIIDLTAERARLEKEIAALDSDIGHVNKKLGNPNFVSRA
APEVVDEQRTKLGEAEAGKVKLQAALARLASL