Protein Info for A4249_RS08390 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 766 TIGR02639: ATP-dependent Clp protease ATP-binding subunit ClpA" amino acids 5 to 741 (737 residues), 1131.3 bits, see alignment E=0 PF02861: Clp_N" amino acids 16 to 62 (47 residues), 43.2 bits, see alignment 3.5e-14 PF00004: AAA" amino acids 218 to 349 (132 residues), 51.4 bits, see alignment E=1.5e-16 amino acids 499 to 614 (116 residues), 44.3 bits, see alignment E=2.4e-14 PF17871: AAA_lid_9" amino acids 357 to 460 (104 residues), 101.6 bits, see alignment E=2.1e-32 PF07724: AAA_2" amino acids 493 to 654 (162 residues), 199.9 bits, see alignment E=3e-62 PF00158: Sigma54_activat" amino acids 496 to 615 (120 residues), 23.6 bits, see alignment E=3.5e-08 PF07728: AAA_5" amino acids 498 to 616 (119 residues), 47.9 bits, see alignment E=1.3e-15 PF10431: ClpB_D2-small" amino acids 660 to 740 (81 residues), 93.1 bits, see alignment E=8.3e-30

Best Hits

Swiss-Prot: 71% identical to CLPA_RHOBL: ClpA homolog protein from Rhodobacter blasticus

KEGG orthology group: K03694, ATP-dependent Clp protease ATP-binding subunit ClpA (inferred from 89% identity to bsb:Bresu_1464)

MetaCyc: 61% identical to ATP-dependent Clp protease ATP-binding subunit ClpA (Escherichia coli K-12 substr. MG1655)
Endopeptidase Clp. [EC: 3.4.21.92]

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpA" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.92

Use Curated BLAST to search for 3.4.21.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160HYX1 at UniProt or InterPro

Protein Sequence (766 amino acids)

>A4249_RS08390 ATP-dependent Clp protease ATP-binding subunit ClpA (Brevundimonas sp. GW460-12-10-14-LB2)
MPSFSRPLEETLHRAVGYANARRHEYATLEHLLLALIDDPDATGVMQACNVDLSALKAAL
TLYVDNDLAALATSDGEDAKPTAGFQRVIQRAVIHVQSSGREEVSGANVLVAIFSERESH
AAYFLQEQDMTRYDAVNFIAHGIAKKAGANEARPAKGASPEEAEDASAVKQGGEALEAYC
VDLNEKSRQGKVDPLIGRQAEVDRSIQILCRRTKNNPLLVGDPGVGKTAIAEGLARKIVN
GEVPDVLKDATIYSLDMGALLAGTRYRGDFEERLKQVVKELENHDNAVLFIDEIHTVIGA
GATSGGAMDASNLLKPALASGTLRCMGSTTYKEYRQHFEKDRALVRRFQKIDVNEPTVED
TVKILKGLKTYYEQHHKVRYTDSAVRTAVELSARYMTDRKLPDKAIDVIDEAGASQMLLT
ESKRKKVIGQKEVEAVIAKMARIPAKSVSKSDTESLRELETDLKRAVFGQEQAIEQVSAA
MKLARAGLRDPNKPIGSFLFSGPTGVGKTEVAKQLAATLGIEMQRFDMSEYMERHTVSRL
IGAPPGYVGHDQGGLLTDAVDQHPHAVVLLDEIEKAHPDVYNILLQVMDNGMLTDAVGKK
VDFRNVILIMTTNAGAADNARASIGFGRGKVEGEDDKAIQRLFAPEFRNRLDAIVAFKPL
QADTIRSVVTKFILQLEAQLADRNITIELTDEAADWLAKNGFDELYGARPLARVIQDSIK
KPLADDILFGRLTRGGHVRVQLKDGKIDFDIMSASAPAKAEEEETA