Protein Info for A4249_RS08195 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: RNA degradosome polyphosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 738 TIGR03705: polyphosphate kinase 1" amino acids 35 to 714 (680 residues), 935.7 bits, see alignment E=8.3e-286 PF13089: PP_kinase_N" amino acids 36 to 141 (106 residues), 124.5 bits, see alignment E=5.6e-40 PF02503: PP_kinase" amino acids 150 to 330 (181 residues), 184.2 bits, see alignment E=5.1e-58 PF17941: PP_kinase_C_1" amino acids 358 to 523 (166 residues), 251.8 bits, see alignment E=6.2e-79 PF13090: PP_kinase_C" amino acids 530 to 700 (171 residues), 208.4 bits, see alignment E=1.4e-65

Best Hits

Swiss-Prot: 63% identical to PPK1_AZOC5: Polyphosphate kinase (ppk) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 88% identity to bsb:Bresu_2212)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.1

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160HZJ8 at UniProt or InterPro

Protein Sequence (738 amino acids)

>A4249_RS08195 RNA degradosome polyphosphate kinase (Brevundimonas sp. GW460-12-10-14-LB2)
MTDAAIHDDTRGEEVPASRAPQLALSEELMASPSRFFNRETSWLAFNERVLEEAANPNHP
LLERLRFLSISANNLDEFFMTRVAGLKGQLRERLRVLSADGLTPAEQLERINIAAGELMA
EQQLRWRQLKKELTVAGIEIVDRQKITKTEKERLEPEFLNQLFAVLTPLAIDPAHPFPFL
PNLGFSLALKLKRKGETKTFYALVPVPTQVRRFWELPTDGRSTPGHKRFITLENVLLLFI
DHLFPGCEVQEKGVLRLIRDSDIEIEEEAEDLVLEFEEALKQRRLGSVVRVKIEASMPVD
LRDFIVGELEAAPQDVVLVDGMLGLAQLSDLIPGGHPDLKFKGYEPRYPERVRDNGGDVF
AAIREKDLLIHHPFESFDVVVQFLRQAARDPNVIAIKQTLYRTSKDSPIVAALIEAAENG
KNVTALVELKARFDEEANLRWARAMERAGVHVVFGFVEYKTHAKVSVVVRREGEGLRTYC
HFGTGNYHPVTAKVYTDLSLFSCDPVLGRDATRVFNYITGYATPDHLEALVVSPLNMKAT
LIELIGKEMSAAAMGKPAAIWVKLNALVDVEIIDALYRASQWGVRIDLVVRGICCLRPGV
PGLSENIRVKSIVGRFLEHSRIVCFANGKDLPHDKAKVFISSADWMTRNLDRRVELMTPI
RNATVHDQVLDQIMVANLKDDAQSWVLDAEGRYTRVTPADPERPFSAHKYFMTNPSLSGR
GRKAKSLPAVLRYERPGR