Protein Info for A4249_RS02595 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: isocitrate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01346: isocitrate lyase
  • amino acids 13 to 240 (228 residues), 314.7 bits, E=4.9e-98
• PF00463: ICL
  • amino acids 14 to 240 (227 residues), 176.7 bits, E=7.4e-56
  • amino acids 244 to 421 (178 residues), 139.2 bits, E=1.7e-44
• PF13714: PEP_mutase
  • amino acids 69 to 223 (155 residues), 50.1 bits, E=2.9e-17

Best Hits

Swiss-Prot: 72% identical to ACEA_BACHD: Isocitrate lyase (aceA) from Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

KEGG orthology group: K01637, isocitrate lyase [EC: 4.1.3.1] (inferred from 96% identity to bsb:Bresu_1695)

MetaCyc: 64% identical to isocitrate lyase subunit (Mycobacterium tuberculosis H37Rv)
Isocitrate lyase. [EC: 4.1.3.1]

Predicted SEED Role

"Isocitrate lyase (EC 4.1.3.1)" in subsystem Serine-glyoxylate cycle (EC 4.1.3.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.3.1

Sequence Analysis Tools

See A0A168NAN7 at UniProt or InterPro

Protein Sequence (426 amino acids)

>A4249_RS02595 isocitrate lyase (Brevundimonas sp. GW460-12-10-14-LB2)
MTTFADLVPSPAGRFDGIERPYTPEDVLRLRGSVPITHTLAERGANRLWQLLHDEPFINA
LGAVTGNQAMQMVRAGLKAIYLSGWQVAADANTAGAMYPDQSLYPANAAPELCRRINRTL
QRADQIEHAEGGAKRDWFVPIVADAEAGFGGPLNSFEIMKAFIEAGAAGVHFEDQLASEK
KCGHLGGKVLIPTQAHERNLVAARLAADVMGTPTITVARTDAESAQLITSDIDERDQPFI
DRDNRTPEGFFRLKEGTGLDHCIARGLSYANIADLLWWETSHPDLDDAKKFAEAIQKAHP
GKLMAYNCSPSFNWKAKLDDATIAKFQRELGAMGYKFQFVTLAGFHSLNNAMFELADGYR
DRGMAAYSELQQREFANEAIGYTATRHQREVGTGYFDQVATVISNGTSSTTALKDSTETA
QFTHAA