Protein Info for A4249_RS02015 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: glycoside hydrolase family 31 protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 978 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details PF13802: Gal_mutarotas_2" amino acids 60 to 224 (165 residues), 42.4 bits, see alignment E=2.4e-14 PF07691: PA14" amino acids 299 to 376 (78 residues), 29.4 bits, see alignment E=1.6e-10 PF01055: Glyco_hydro_31_2nd" amino acids 428 to 771 (344 residues), 283 bits, see alignment E=8.7e-88 PF21365: Glyco_hydro_31_3rd" amino acids 780 to 866 (87 residues), 99.2 bits, see alignment E=3.9e-32 PF17137: DUF5110" amino acids 882 to 950 (69 residues), 83.4 bits, see alignment 2.3e-27

Best Hits

KEGG orthology group: K01811, putative family 31 glucosidase (inferred from 55% identity to cse:Cseg_3576)

Predicted SEED Role

"Maltodextrin glucosidase (EC 3.2.1.20)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.20

Use Curated BLAST to search for 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168N7N3 at UniProt or InterPro

Protein Sequence (978 amino acids)

>A4249_RS02015 glycoside hydrolase family 31 protein (Brevundimonas sp. GW460-12-10-14-LB2)
MIPTLARRLFLAALMTAPLAACAGQGLMNGASAGAIQQAAFERTATGIVVTPAEGAARRV
RLEVYGPETIRVTATENADLELPASLMVTAQPSTTTPFQVETRGDLVVLKTAEATAEVSR
ETGAVRFLDENGQEVLKERAGSRELSPVTVEDQKGDHRFLATRQVWESPADEAFYGLGQH
QQGVMNYKGHDVQLAQHNIDVGIPLVVSSRNYGVLWDNNSITRFGDPRPYGMIADQFIVR
DATGRFGGLTASYYQNDQLKVQRQETEVNYEFIREWSRWPEGFKSQPGQPVQTGDMGKPG
QDGVKVVWEGTLETAAAGLHKFRLYSSGYAKVWIDGRMVEDNWRQNWNPFDHDFQVQMAA
NTRVPIKIEWIPEDGYISLRHQDPLPAADLGALSLASEVGTAIDYYYIGGDDLDGVISNY
RGLTGKSVMLPRWAYGFWQSRQRYNTQDEVTGVVRRYRELGLPIDNIVQDWFYWPENAWG
SHQFDPARFPNPTQMVKDVHDMNANIIISVWPKFYPTTDHYKQLDAIGGMYTRNVDVGAR
DWVGPGYANSFYDPYLPAARDLYWKQMSDSFKGMNFDGWWLDSVEPDMHSNLDIPERTLR
MGPTAIGPGAYVFNSYPLVNAENVYNNFRREKGDERTYILTRSAWGGLQRTGSTLWSGDV
VARWDNLKDQISAGVNTGLSGLANWTHDIGGFAVEARYSNPAGMKPEDKAEWDELNLRWF
QFGAWSPIFRSHGEFPLREVYNLAQPGTSLYDAYAQQMRTRYRLMPYIYSLAGDVYQRDA
TIMRGLVMDFPSDLKARDINDQYLFGHALLVAPVTEYKARSRSVYFPQGADWYDMLTGQK
FAGGRSVTVQAPEDKIPVFVRAGSILPTGPAIQYTAEKLDGPLTLIVYTGANGSFSLYED
QGTTYAYERGEFSNIPMTWNDATGVLTIGARTGSYPTMPANRTINVRFVSGPGADVGNFD
AAPARTIQYNGQAVEVRR