Protein Info for A4249_RS01050 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: N-acetylglutaminylglutamine synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 589 TIGR03103: GNAT-family acetyltransferase TIGR03103" amino acids 36 to 577 (542 residues), 909 bits, see alignment E=5.1e-278 PF00583: Acetyltransf_1" amino acids 128 to 244 (117 residues), 56.3 bits, see alignment E=1.1e-18 PF13508: Acetyltransf_7" amino acids 156 to 245 (90 residues), 42.1 bits, see alignment E=2.8e-14 PF08445: FR47" amino acids 187 to 246 (60 residues), 31.7 bits, see alignment 3.7e-11 PF13673: Acetyltransf_10" amino acids 190 to 248 (59 residues), 31.6 bits, see alignment 4.5e-11

Best Hits

KEGG orthology group: None (inferred from 67% identity to mes:Meso_2468)

MetaCyc: 65% identical to N-acetylglutaminylglutamine synthase (Sinorhizobium meliloti 1021)
RXN-13455

Predicted SEED Role

"Cyanophycin synthase (EC 6.3.2.29)(EC 6.3.2.30)" in subsystem Cyanophycin Metabolism (EC 6.3.2.29, EC 6.3.2.30)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.29 or 6.3.2.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168N195 at UniProt or InterPro

Protein Sequence (589 amino acids)

>A4249_RS01050 N-acetylglutaminylglutamine synthetase (Brevundimonas sp. GW460-12-10-14-LB2)
MASDIRPERSKAHRLKRLRHEGLKPPVQSGEGPTPDAVLDCGWGRLLFAQTFETAEPLVE
ALRAEGPDRRDIAFYVRNPHVLLASAPQELFLDPSHTYRLDLATYRTSRRQPRGFTVRRL
TSETDAQAVNDIYAKRKMVPVPPDFFWSHRDDRTLTYFVAEDTVTGAVIGTVTGVNHARA
FQDPECGSSLWCLAVDPQATQPGVGEALVRRLAEHFKTQGLAHMDLSVMHDNDQAIALYE
KLGFRRVSFFGVKRKNPINEALFVGPTDDHDLNPYARIIVDEARRRGIHTEIIDGEGGLF
RLNWGGRSVRCRESLSELTSAVALSICDDKRMTRRIVEAAGVRVPERIDPENPSAIDAAL
TKFGALVVKPARGEQGQGVAVGLTTMSEVQTALVRARCICSEVLVEEQVQGEDLRLVVID
YKVVACALRRPPRVVGDGRSTLRTLIEHQSRRRAAATGGESTIPIDAETERTLAEAGYRL
DDVAAEGTGILVRKAANLHLGGTIHDVTDEVHPALIRAAVAAARAIDIPVTGIDLMVKSP
REPDYAFIEANERPGLANHEPQPTAERFVDLLFPLSAPAAARTFPRSEA