Protein Info for b4301 in Escherichia coli BW25113

Name: sgcE
Annotation: ribulose-phosphate 3-epimerase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00834: Ribul_P_3_epim
  • amino acids 3 to 195 (193 residues), 252.3 bits, E=1.4e-79

Best Hits

Swiss-Prot: 100% identical to SGCE_ECOLI: Protein SgcE (sgcE) from Escherichia coli (strain K12)

KEGG orthology group: K14587, protein sgcE [EC: 5.1.3.-] (inferred from 100% identity to eco:b4301)

Predicted SEED Role

"Ribulose-phosphate 3-epimerase (EC 5.1.3.1)" in subsystem Calvin-Benson cycle or Conserved gene cluster associated with Met-tRNA formyltransferase or Pentose phosphate pathway or Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization (EC 5.1.3.1)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (17/17 steps found)
• heterolactic fermentation (16/18 steps found)
• pentose phosphate pathway (8/8 steps found)
• Bifidobacterium shunt (13/15 steps found)
• Rubisco shunt (9/10 steps found)
• pentose phosphate pathway (non-oxidative branch) I (5/5 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
• pentose phosphate pathway (partial) (3/3 steps found)
• pentose phosphate pathway (non-oxidative branch) II (5/6 steps found)
• Calvin-Benson-Bassham cycle (10/13 steps found)
• formaldehyde assimilation II (assimilatory RuMP Cycle) (7/9 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• oxygenic photosynthesis (11/17 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Nucleotide sugars metabolism
• Pentose and glucuronate interconversions
• Pentose phosphate pathway
• Polyketide sugar unit biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.-, 5.1.3.1

Use Curated BLAST to search for 5.1.3.- or 5.1.3.1

Sequence Analysis Tools

See P39362 at UniProt or InterPro

Protein Sequence (210 amino acids)

>b4301 ribulose-phosphate 3-epimerase (NCBI) (Escherichia coli BW25113)
MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNITFGMKTVQAVARQTPHP
LSFHFMLARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEIRHTGARAGLVFNPATPID
AWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTAFPQTECWADGGITLAAAQQL
AAAGAQHMVIGRALFSSSDYRATLAQFATL