Protein Info for b4278 in Escherichia coli BW25113

Name: insG
Annotation: KpLE2 phage-like element; IS4 predicted transposase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 442 PF13006: Nterm_IS4" amino acids 18 to 109 (92 residues), 114.3 bits, see alignment E=2.5e-37 PF01609: DDE_Tnp_1" amino acids 126 to 352 (227 residues), 108.9 bits, see alignment E=3e-35

Best Hits

Swiss-Prot: 100% identical to INSG_ECOLI: Transposase InsG for insertion sequence element IS4 (insG) from Escherichia coli (strain K12)

KEGG orthology group: K07495, putative transposase (inferred from 100% identity to eco:b4278)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P03835 at UniProt or InterPro

Protein Sequence (442 amino acids)

>b4278 KpLE2 phage-like element; IS4 predicted transposase (NCBI) (Escherichia coli BW25113)
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVG
MALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATP
HPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAA
AFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQY
EEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPG
GEMGDLYSHRWEIELGYREIKQTMQRSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRERAF
PRVVKERPWKYPTAPKKSQSVA