Protein Info for b4238 in Escherichia coli BW25113

Name: nrdD
Annotation: anaerobic ribonucleoside triphosphate reductase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03477: ATP-cone
  • amino acids 4 to 89 (86 residues), 77.6 bits, E=1.3e-25
• TIGR02487: anaerobic ribonucleoside-triphosphate reductase
  • amino acids 115 to 705 (591 residues), 708.7 bits, E=2.9e-217
• PF13597: NRDD
  • amino acids 116 to 705 (590 residues), 658.8 bits, E=1.2e-201
• PF01228: Gly_radical
  • amino acids 581 to 686 (106 residues), 108.3 bits, E=4.2e-35

Best Hits

Swiss-Prot: 100% identical to NRDD_ECOLI: Anaerobic ribonucleoside-triphosphate reductase (nrdD) from Escherichia coli (strain K12)

KEGG orthology group: K00527, ribonucleoside-triphosphate reductase [EC: 1.17.4.2] (inferred from 100% identity to ecv:APECO1_2154)

MetaCyc: 100% identical to anaerobic ribonucleoside-triphosphate reductase (Escherichia coli K-12 substr. MG1655)
RIBONUCLEOSIDE-TRIP-REDUCT-RXN [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]

Predicted SEED Role

"Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)" in subsystem Ribonucleotide reduction (EC 1.17.4.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (7/7 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.98.6 or 1.17.4.2

Sequence Analysis Tools

See P28903 at UniProt or InterPro

Protein Sequence (712 amino acids)

>b4238 anaerobic ribonucleoside triphosphate reductase (NCBI) (Escherichia coli BW25113)
MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAAVVSEQMQGRNQVDINE
IQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEIRGLVEQTNASLLNENANK
DSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIHYHDLDYSPFFPMFNCMLIDL
KGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASHIYGGTTINRIDEVLAPFVTASY
NKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEYEVNTLHTANGQTPFVTFGFGLGTS
WESRLIQESILRNRIAGLGKNRKTAVFPKLVFAIRDGLNHKKGDPNYDIKQLALECASKR
MYPDILNYDQVVKVTGSFKTPMGCRSFLGVWENENGEQIHDGRNNLGVISLNLPRIALEA
KGDEATFWKLLDERLVLARKALMTRIARLEGVKARVAPILYMEGACGVRLNADDDVSEIF
KNGRASISLGYIGIHETINALFGGEHVYDNEQLRAKGIAIVERLRQAVDQWKEETGYGFS
LYSTPSENLCDRFCRLDTAEFGVVPGVTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPL
ANGGFICYGEYPNIQHNLKALEDVWDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGF
TCPKCGNHDASRVSVTRRVCGYLGSPDARPFNAGKQEEVKRRVKHLGNGQIG