Protein Info for b4235 in Escherichia coli BW25113

Name: pmbA
Annotation: predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 PF01523: PmbA_TldD_1st" amino acids 36 to 100 (65 residues), 50.6 bits, see alignment E=2.8e-17 PF19290: PmbA_TldD_2nd" amino acids 128 to 234 (107 residues), 75.5 bits, see alignment E=7e-25 PF19289: PmbA_TldD_3rd" amino acids 242 to 449 (208 residues), 248.7 bits, see alignment E=5.6e-78

Best Hits

Swiss-Prot: 100% identical to PMBA_ECOLI: Metalloprotease PmbA (pmbA) from Escherichia coli (strain K12)

KEGG orthology group: K03592, PmbA protein (inferred from 100% identity to eco:b4235)

MetaCyc: 100% identical to metalloprotease subunit TldE (Escherichia coli)
3.4.24.-

Predicted SEED Role

"TldE protein, part of TldE/TldD proteolytic complex"

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AFK0 at UniProt or InterPro

Protein Sequence (450 amino acids)

>b4235 predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI) (Escherichia coli BW25113)
MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEF
NSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARYTSPDPCAGVADKELLAFD
APDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEGGSFNSHYGVKVFGNSHGMLQ
GYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQTPEWVGADCARRTLSRLSPRKLS
TMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFLLDSLGKQILPDWLTIEEHPHLLKG
LASTPFDSEGVRTERRDIIKDGILTQWLLTSYSARKLGLKSTGHAGGIHNWRIAGQGLSF
EQMLKEMGTGLVVTELMGQGVSAITGDYSRGAAGFWVENGEIQYPVSEITIAGNLKDMWR
NIVTVGNDIETRSNIQCGSVLLPEMKIAGQ