Protein Info for b4213 in Escherichia coli BW25113

Name: cpdB
Annotation: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 647 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR01390: 2',3'-cyclic-nucleotide 2'-phosphodiesterase" amino acids 22 to 647 (626 residues), 1248.2 bits, see alignment E=0 PF00149: Metallophos" amino acids 24 to 260 (237 residues), 54.8 bits, see alignment E=1.8e-18 PF02872: 5_nucleotid_C" amino acids 370 to 550 (181 residues), 69.5 bits, see alignment E=3.6e-23

Best Hits

Swiss-Prot: 100% identical to CPDB_ECOLI: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (cpdB) from Escherichia coli (strain K12)

KEGG orthology group: K01119, 2',3'-cyclic-nucleotide 2'-phosphodiesterase [EC: 3.1.4.16] (inferred from 100% identity to eco:b4213)

MetaCyc: 100% identical to 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (Escherichia coli K-12 substr. MG1655)
2',3'-cyclic-nucleotide 2'-phosphodiesterase. [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3'-nucleotidase. [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]

Predicted SEED Role

"2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)" in subsystem Purine conversions (EC 3.1.4.16)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.6 or 3.1.4.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P08331 at UniProt or InterPro

Protein Sequence (647 amino acids)

>b4213 bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein (NCBI) (Escherichia coli BW25113)
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDTATEKFGLVRTASLIN
DARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYKALNTLDYTVGTLGNHEFN
YGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDTEVVDKDGKKQTLKIGYIGVV
PPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGADVVVVLAHSGLSADPYKVMAENS
VYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIAKGTLNGVPAVMPGMWGDHLGVVDL
QLSNDSGKWQVTQAKAEARPIYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSA
DNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPAS
YVEVEKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIDPNSTKPQSLIN
WDGFRTYNFDVIDGVNYQIDVTQPARYDGECQMINANAERIKNLTFNGKPIDPNAMFLVA
TNNYRAYGGKFAGTGDSHIAFASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAPIAG
DKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK