Protein Info for b4208 in Escherichia coli BW25113

Name: cycA
Annotation: D-alanine/D-serine/glycine transporter (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 470 transmembrane" amino acids 28 to 45 (18 residues), see Phobius details amino acids 51 to 73 (23 residues), see Phobius details amino acids 106 to 130 (25 residues), see Phobius details amino acids 136 to 154 (19 residues), see Phobius details amino acids 165 to 187 (23 residues), see Phobius details amino acids 211 to 232 (22 residues), see Phobius details amino acids 252 to 274 (23 residues), see Phobius details amino acids 286 to 308 (23 residues), see Phobius details amino acids 345 to 364 (20 residues), see Phobius details amino acids 370 to 397 (28 residues), see Phobius details amino acids 417 to 435 (19 residues), see Phobius details amino acids 441 to 460 (20 residues), see Phobius details PF00324: AA_permease" amino acids 27 to 468 (442 residues), 451.8 bits, see alignment E=2.7e-139 PF13520: AA_permease_2" amino acids 31 to 445 (415 residues), 161.2 bits, see alignment E=4.1e-51

Best Hits

Swiss-Prot: 100% identical to CYCA_ECO57: D-serine/D-alanine/glycine transporter (cycA) from Escherichia coli O157:H7

KEGG orthology group: K11737, D-serine/D-alanine/glycine transporter (inferred from 100% identity to eco:b4208)

MetaCyc: 100% identical to D-serine/alanine/glycine:H+symporter (Escherichia coli K-12 substr. MG1655)
RXN0-5130; RXN0-5201; RXN0-5202; RXN0-5203; TRANS-RXN-62A; TRANS-RXN-62B

Predicted SEED Role

"D-serine/D-alanine/glycine transporter" in subsystem Glycine and Serine Utilization or Pyruvate Alanine Serine Interconversions

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AAE0 at UniProt or InterPro

Protein Sequence (470 amino acids)

>b4208 D-alanine/D-serine/glycine transporter (NCBI) (Escherichia coli BW25113)
MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMGSGKTISLAGPSIIFVY
MIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYFTGWTYWFCWVVTGMADVV
AITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEMEFWFAMIKIVAIVSLIVVGL
VMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGFQIAVFAFVGIELVGTTAAETKD
PEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSVVPEKSPFVELFVLVGLPAAASVIN
FVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKAFAKLSKRAVPAKGLTFSCICLLGGVV
MLYVNPSVIGAFTMITTVSAILFMFVWTIILCSYLVYRKQRPHLHEKSIYKMPLGKLMCW
VCMAFFVFVVVLLTLEDDTRQALLVTPLWFIALGLGWLFIGKKRAAELRK