Protein Info for b4187 in Escherichia coli BW25113

Name: aidB
Annotation: putative acyl coenzyme A dehydrogenase (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 541 PF18158: AidB_N" amino acids 10 to 167 (158 residues), 186.8 bits, see alignment E=5.3e-59 PF02770: Acyl-CoA_dh_M" amino acids 181 to 275 (95 residues), 62.8 bits, see alignment E=5.6e-21 PF00441: Acyl-CoA_dh_1" amino acids 285 to 438 (154 residues), 105.9 bits, see alignment E=4.7e-34 PF08028: Acyl-CoA_dh_2" amino acids 309 to 420 (112 residues), 32.3 bits, see alignment E=2.2e-11

Best Hits

Swiss-Prot: 100% identical to AIDB_ECOLI: Putative acyl-CoA dehydrogenase AidB (aidB) from Escherichia coli (strain K12)

KEGG orthology group: K09456, putative acyl-CoA dehydrogenase (inferred from 100% identity to eco:b4187)

MetaCyc: 100% identical to isovaleryl-CoA dehydrogenase and DNA-binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)
RXN0-2301 [EC: 1.3.8.4]

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7

Use Curated BLAST to search for 1.3.8.4 or 1.3.8.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P33224 at UniProt or InterPro

Protein Sequence (541 amino acids)

>b4187 putative acyl coenzyme A dehydrogenase (VIMSS) (Escherichia coli BW25113)
MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLASIGQQLGTAESLELGR
LANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHNLAWEEDARSGAFVARAAR
FMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPLLSDRYDSHLLPGGQKRGLLI
GMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFSVPQSDAHLVLAQTAGGLSCFFV
PRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDAIGWLLGLEGEGIRLILKMGGMTRF
DCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQPLMRHVLSRMALQLEGQTALLFRLAR
AWDRRADAKEALWARLFTPAAKFVICKRGMPFVAEAMEVLGGIGYCEESELPRLYREMPV
NSIWEGSGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQQLRKPAEE
LGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQNDLLLRATGGVC
V