Protein Info for b4186 in Escherichia coli BW25113

Name: yjfC
Annotation: predicted synthetase/amidase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03738: GSP_synth
  • amino acids 14 to 385 (372 residues), 500.4 bits, E=1.8e-154

Best Hits

Swiss-Prot: 100% identical to YJFC_ECOLI: Putative acid--amine ligase YjfC (yjfC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b4186)

MetaCyc: 100% identical to peptide--spermidine ligase YjfC (Escherichia coli K-12 substr. MG1655)
6.3.1.-

Predicted SEED Role

"Similarity with glutathionylspermidine synthase (EC 6.3.1.8), group 1" in subsystem Glutathionylspermidine and Trypanothione (EC 6.3.1.8)

MetaCyc Pathways

• glutathionylspermidine biosynthesis (1/1 steps found)

KEGG Metabolic Maps

• Glutathione metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.8

Use Curated BLAST to search for 6.3.1.8

Sequence Analysis Tools

See P33222 at UniProt or InterPro

Protein Sequence (387 amino acids)

>b4186 predicted synthetase/amidase (NCBI) (Escherichia coli BW25113)
MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMC
LEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAD
TPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLISRFSELYSREPFYFCCCQDT
DEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRD
DNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFDGEKPQIAAGESYV
RKPIYSREGGNVTIFDGKNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDE
ACGMGIREDNTLITKDTSRFVPHYIAG