Protein Info for b3844 in Escherichia coli BW25113

Name: fre
Annotation: NAD(P)H-flavin reductase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 233 PF00970: FAD_binding_6" amino acids 6 to 94 (89 residues), 25.3 bits, see alignment E=1.7e-09 PF00175: NAD_binding_1" amino acids 108 to 211 (104 residues), 91.9 bits, see alignment E=4e-30

Best Hits

Swiss-Prot: 100% identical to FRE_ECO57: NAD(P)H-flavin reductase (fre) from Escherichia coli O157:H7

KEGG orthology group: K05368, aquacobalamin reductase / NAD(P)H-flavin reductase [EC: 1.16.1.3 1.5.1.29] (inferred from 100% identity to eco:b3844)

MetaCyc: 100% identical to NAD(P)H-flavin reductase (Escherichia coli K-12 substr. MG1655)
RXN-12445 [EC: 1.5.1.41]; FMNREDUCT-RXN [EC: 1.5.1.41, 1.5.1.39]; RXN-8506 [EC: 1.5.1.41, 1.5.1.39, 1.5.1.37]; Flavin reductase. [EC: 1.5.1.41, 1.5.1.39, 1.5.1.37, 1.5.1.30]

Predicted SEED Role

"NAD(P)H-flavin reductase (EC 1.5.1.29) (EC 1.16.1.3)" in subsystem Ubiquinone Biosynthesis (EC 1.16.1.3, EC 1.5.1.29)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.29

Use Curated BLAST to search for 1.16.1.3 or 1.5.1.29 or 1.5.1.30 or 1.5.1.37 or 1.5.1.39 or 1.5.1.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AEN1 at UniProt or InterPro

Protein Sequence (233 amino acids)

>b3844 NAD(P)H-flavin reductase (NCBI) (Escherichia coli BW25113)
MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDERDKRPFSMASTPDEKGF
IELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDEERPMILIAGGTGFSYARS
ILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHPGLQVVPVVEQPEAGWRGRTG
TVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSERNAREDRLFGDAFAFI