Protein Info for b3665 in Escherichia coli BW25113

Name: ade
Annotation: cryptic adenine deaminase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 588 TIGR01178: adenine deaminase" amino acids 31 to 584 (554 residues), 840.6 bits, see alignment E=2.6e-257 PF01979: Amidohydro_1" amino acids 82 to 362 (281 residues), 134.7 bits, see alignment E=7.4e-43 PF13382: Adenine_deam_C" amino acids 434 to 579 (146 residues), 185.3 bits, see alignment E=9.7e-59

Best Hits

Swiss-Prot: 100% identical to ADEC_ECODH: Adenine deaminase (ade) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K01486, adenine deaminase [EC: 3.5.4.2] (inferred from 100% identity to eco:b3665)

MetaCyc: 100% identical to adenine deaminase (Escherichia coli K-12 substr. MG1655)
Adenine deaminase. [EC: 3.5.4.2]; Catalase. [EC: 3.5.4.2, 1.11.1.21, 1.11.1.6]

Predicted SEED Role

"Adenine deaminase (EC 3.5.4.2)" in subsystem Purine conversions (EC 3.5.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.11.1.6

Use Curated BLAST to search for 1.11.1.21 or 1.11.1.6 or 3.5.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P31441 at UniProt or InterPro

Protein Sequence (588 amino acids)

>b3665 cryptic adenine deaminase (NCBI) (Escherichia coli BW25113)
MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIA
GVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICDPH
EIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRDHPQV
TGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIENCHESYQL
EEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIAHEGHIDAL
IRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDARKVTVQQVLVK
GEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGKRYRVIDVIHNELIT
HSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFGLNEGALAATVSHDSHNIVV
IGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMSTDTAQSLAEQIDALKAA
ARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFTTLEVTE