Protein Info for b3662 in Escherichia coli BW25113

Name: nepI
Annotation: predicted transporter (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details amino acids 56 to 75 (20 residues), see Phobius details amino acids 86 to 105 (20 residues), see Phobius details amino acids 111 to 133 (23 residues), see Phobius details amino acids 145 to 165 (21 residues), see Phobius details amino acids 176 to 195 (20 residues), see Phobius details amino acids 217 to 239 (23 residues), see Phobius details amino acids 251 to 275 (25 residues), see Phobius details amino acids 282 to 301 (20 residues), see Phobius details amino acids 307 to 330 (24 residues), see Phobius details amino acids 342 to 363 (22 residues), see Phobius details amino acids 369 to 389 (21 residues), see Phobius details PF07690: MFS_1" amino acids 25 to 335 (311 residues), 130.8 bits, see alignment E=2.9e-42

Best Hits

Swiss-Prot: 100% identical to NEPI_SHIFL: Purine ribonucleoside efflux pump NepI (nepI) from Shigella flexneri

KEGG orthology group: K03445, MFS transporter, DHA1 family, purine ribonucleoside efflux pump (inferred from 100% identity to eco:b3662)

MetaCyc: 100% identical to purine ribonucleoside exporter (Escherichia coli K-12 substr. MG1655)
RXN0-18; RXN0-22

Predicted SEED Role

"Putative transport protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0ADL1 at UniProt or InterPro

Protein Sequence (396 amino acids)

>b3662 predicted transporter (RefSeq) (Escherichia coli BW25113)
MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLTPMAQDLGISEGVAGQS
VTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLVSFANSFSLLLIGRACLGL
ALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIAAPLGSFLGELIGWRNVFNAA
AVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGVMAGMIAIFMSFAGQFAFFTYIR
PVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFILKRSVKLALAGAPLILAVSALVLTL
WGSDKIVATGVAIIWGLTFALVPVGWSTWITRSLADQAEKAGSIQVAVIQLANTCGAAIG
GYALDNIGLTSPLMLSGTLMLLTALLVTAKVKMKKS