Protein Info for b3650 in Escherichia coli BW25113

Name: spoT
Annotation: bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 702 PF13328: HD_4" amino acids 26 to 175 (150 residues), 180.6 bits, see alignment E=5.6e-57 TIGR00691: RelA/SpoT family protein" amino acids 26 to 699 (674 residues), 976.9 bits, see alignment E=2.8e-298 PF01966: HD" amino acids 45 to 144 (100 residues), 52.8 bits, see alignment E=1.4e-17 PF04607: RelA_SpoT" amino acids 235 to 345 (111 residues), 144.7 bits, see alignment E=3.8e-46 PF02824: TGS" amino acids 388 to 447 (60 residues), 76.9 bits, see alignment 3e-25 PF19296: RelA_AH_RIS" amino acids 459 to 541 (83 residues), 36 bits, see alignment E=2.5e-12 PF13291: ACT_4" amino acids 622 to 699 (78 residues), 59.1 bits, see alignment E=1.7e-19

Best Hits

Swiss-Prot: 100% identical to SPOT_ECO57: Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (spoT) from Escherichia coli O157:H7

KEGG orthology group: K01139, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [EC: 3.1.7.2] (inferred from 100% identity to ecq:ECED1_4334)

MetaCyc: 100% identical to bifunctional (p)ppGpp synthase/hydrolase SpoT (Escherichia coli K-12 substr. MG1655)
Guanosine-3',5'-bis(diphosphate) 3'-diphosphatase. [EC: 3.1.7.2]; 3.1.7.2 [EC: 3.1.7.2]; GTP diphosphokinase. [EC: 3.1.7.2, 2.7.6.5]

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AG24 at UniProt or InterPro

Protein Sequence (702 amino acids)

>b3650 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI) (Escherichia coli BW25113)
MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMK
LDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFR
KMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHIKT
ELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
YSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKA
NGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQ
SLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIGHA
CVGARVDRQPYPLSQPLTSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
DSVSLGRRLLNHALGGSRKLNEIPQENIQRELDRMKLATLDDLLAEIGLGNAMSVVVAKN
LQHGDASIPPATQSHGHLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCR
NIRGYQKEPEKFMAVEWDKETAQEFITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTE
EKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN