Protein Info for b3639 in Escherichia coli BW25113

Name: dfp
Annotation: flavoprotein affecting synthesis of DNA and pantothenate metabolism (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00521: phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase
  • amino acids 3 to 397 (395 residues), 550.1 bits, E=1.5e-169
• PF02441: Flavoprotein
  • amino acids 6 to 176 (171 residues), 113.5 bits, E=7.6e-37
• PF04127: DFP
  • amino acids 187 to 371 (185 residues), 249.5 bits, E=2.1e-78

Best Hits

Swiss-Prot: 100% identical to COABC_ECOL6: Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K13038, phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase [EC: 4.1.1.36 6.3.2.5] (inferred from 100% identity to eco:b3639)

MetaCyc: 100% identical to fused 4'-phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase (Escherichia coli K-12 substr. MG1655)
Phosphopantothenate--cysteine ligase. [EC: 6.3.2.5]; Phosphopantothenoylcysteine decarboxylase. [EC: 6.3.2.5, 4.1.1.36]

Predicted SEED Role

"Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5)" in subsystem Coenzyme A Biosynthesis (EC 4.1.1.36, EC 6.3.2.5)

MetaCyc Pathways

• superpathway of coenzyme A biosynthesis I (bacteria) (9/9 steps found)
• superpathway of coenzyme A biosynthesis III (mammals) (5/5 steps found)
• coenzyme A biosynthesis I (bacteria) (4/4 steps found)
• coenzyme A biosynthesis II (eukaryotic) (4/4 steps found)
• coenzyme A biosynthesis III (archaea) (3/4 steps found)
• taurine biosynthesis II (1/4 steps found)

KEGG Metabolic Maps

• Pantothenate and CoA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.36 or 6.3.2.5

Sequence Analysis Tools

See P0ABQ0 at UniProt or InterPro

Protein Sequence (406 amino acids)

>b3639 flavoprotein affecting synthesis of DNA and pantothenate metabolism (VIMSS) (Escherichia coli BW25113)
MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAKAFITPLSLQAVSGYPV
SDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGMANDLVSTICLATPAPVAV
LPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGDIGPGRMLDPLTIVDMAVAHF
SPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGFAIAAAAARRGANVTLVSGPVSL
PTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAAVADYRAATVAPEKIKKQATQGDEL
TIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNVEEYARQKRIRKNLDLICANDVSQPTQ
GFNSDNNALHLFWQDGDKVLPLERKELLGQLLLDEIVTRYDEKNRR