Protein Info for b3629 in Escherichia coli BW25113
Name: rfaS
Annotation: lipopolysaccharide core biosynthesis protein (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
No protein families (PFam or TIGRFam), signal peptides, or transmembrane helices were found in this protein.
Best Hits
Swiss-Prot: 100% identical to RFAS_ECOLI: Lipopolysaccharide core biosynthesis protein RfaS (rfaS) from Escherichia coli (strain K12)
KEGG orthology group: K12986, 1,5-rhamnosyltransferase [EC: 2.4.1.-] (inferred from 100% identity to eco:b3629)Predicted SEED Role
"Lipopolysaccharide core biosynthesis protein RfaS" in subsystem LOS core oligosaccharide biosynthesis
KEGG Metabolic Maps
- Anthocyanin biosynthesis
- Carotenoid biosynthesis - General
- Flavone and flavonol biosynthesis
- Flavonoid biosynthesis
- Fructose and mannose metabolism
- Glycerolipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - lacto and neolacto series
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
- High-mannose type N-glycan biosynthesis
- Keratan sulfate biosynthesis
- Lipopolysaccharide biosynthesis
- N-Glycan biosynthesis
- O-Glycan biosynthesis
- O-Mannosyl glycan biosynthesis
- Peptidoglycan biosynthesis
- Sphingolipid metabolism
- Zeatin biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.4.1.-
Use Curated BLAST to search for 2.4.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P27126 at UniProt or InterPro
Protein Sequence (311 amino acids)
>b3629 lipopolysaccharide core biosynthesis protein (NCBI) (Escherichia coli BW25113) MTIYFINWVADYELKMIQYLKKKYKIKNITTPKKYNWINKKISKIGMDNAWLGRLFIKHY LNDIKKDDIIIINDSVVNKGINKQILKNINCHKVLLLRNTVGEDFILDNANYFDIIYDFE HRFIGNEKIKAIEQFFPIGMDEIRNYSLSDKNNSQPICFFLGRDKGRLQIINELAERLTT LGCKLDFNVVKDKTSSTTSKYLIEKQISYEENIRRTLNANIIVDITKENQSGWTLRILEA LFFNKKLITNNINVFGSEIYSESRFFIIGHDDWDKLEYFINSSVKPMDYDSLYKFSPDKM MSTIVSDFIDK