Protein Info for b3617 in Escherichia coli BW25113

Name: kbl
Annotation: 2-amino-3-ketobutyrate coenzyme A ligase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 TIGR01822: glycine C-acetyltransferase" amino acids 5 to 397 (393 residues), 746.5 bits, see alignment E=3e-229 PF00155: Aminotran_1_2" amino acids 43 to 387 (345 residues), 264.7 bits, see alignment E=1.5e-82 PF01041: DegT_DnrJ_EryC1" amino acids 88 to 217 (130 residues), 21.1 bits, see alignment E=1.7e-08

Best Hits

Swiss-Prot: 100% identical to KBL_ECO57: 2-amino-3-ketobutyrate coenzyme A ligase (kbl) from Escherichia coli O157:H7

KEGG orthology group: K00639, glycine C-acetyltransferase [EC: 2.3.1.29] (inferred from 100% identity to eco:b3617)

MetaCyc: 100% identical to 2-amino-3-ketobutyrate CoA ligase (Escherichia coli K-12 substr. MG1655)
Glycine C-acetyltransferase. [EC: 2.3.1.29]

Predicted SEED Role

"2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)" in subsystem Glycine Biosynthesis or Glycine and Serine Utilization (EC 2.3.1.29)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AB77 at UniProt or InterPro

Protein Sequence (398 amino acids)

>b3617 2-amino-3-ketobutyrate coenzyme A ligase (NCBI) (Escherichia coli BW25113)
MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHVINFCANNYLGLANHPD
LIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGMEDAILYSSCFDANGGLFE
TLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQELEARLKEAREAGARHVLIAT
DGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFVGENGRGSHEYCDVMGRVDIITG
TLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNSLAPAIVAASIKVLEMVEAGSELRD
RLWANARQFREQMSAAGFTLAGADHAIIPVMLGDAVVAQKFARELQKEGIYVTGFFYPVV
PKGQARIRTQMSAAHTPEQITRAVEAFTRIGKQLGVIA