Protein Info for b3529 in Escherichia coli BW25113

Name: yhjK
Annotation: predicted diguanylate cyclase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 662 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 154 to 179 (26 residues), see Phobius details PF17154: GAPES3" amino acids 36 to 155 (120 residues), 155.8 bits, see alignment E=7.1e-50 PF00990: GGDEF" amino acids 247 to 394 (148 residues), 42 bits, see alignment E=1.3e-14 PF00563: EAL" amino acids 415 to 648 (234 residues), 205.3 bits, see alignment E=1.5e-64

Best Hits

Swiss-Prot: 100% identical to PDEK_ECOLI: Probable cyclic di-GMP phosphodiesterase PdeK (pdeK) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b3529)

MetaCyc: 100% identical to c-di-GMP phosphodiesterase PdeK (Escherichia coli K-12 substr. MG1655)
Cyclic-guanylate-specific phosphodiesterase. [EC: 3.1.4.52]

Predicted SEED Role

"Protein yhjK"

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.52

Use Curated BLAST to search for 3.1.4.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P37649 at UniProt or InterPro

Protein Sequence (662 amino acids)

>b3529 predicted diguanylate cyclase (RefSeq) (Escherichia coli BW25113)
MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTATQLESIARSVREPLSSA
ILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIPERPVPVMVTRLFELPVQI
SLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLVTIYLLLSLILTVAISWCINR
LILHPLRNIARELNAIPAKELVGHQLALPRLHQDDEIGMLVRSYNLNQQLLQRHYEEQNE
NAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIITCETLRDTAGVLKEAQREILLLTLV
EKLKSVLSPRMILAQISGYDFAVIANGVQEPWHAITLGQQVLTIMSERLPIERIQLRPHC
SIGVAMFYGDLTAEQLYSRAISAAFTARHKGKNQIQFFDPQQMEAAQKRLTEESDILNAL
ENHQFAIWLQPQVEMTSGKLVSAEVLLRIQQPDGSWDLPDGLIDRIECCGLMVTVGHWVL
EESCRLLAAWQERGIMLPLSVNLSALQLMHPNMVADMLELLTRYRIQPGTLILEVTESRR
IDDPHAAVAILRPLRNAGVRVALDDFGMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPGD
SSMIAAIIMLAQSLNLQMIAEGVETEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLE
EK