Protein Info for b3469 in Escherichia coli BW25113

Name: zntA
Annotation: zinc, cobalt and lead efflux system (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 732 transmembrane" amino acids 128 to 144 (17 residues), see Phobius details amino acids 150 to 168 (19 residues), see Phobius details amino acids 180 to 222 (43 residues), see Phobius details amino acids 353 to 375 (23 residues), see Phobius details amino acids 385 to 410 (26 residues), see Phobius details amino acids 685 to 702 (18 residues), see Phobius details amino acids 708 to 729 (22 residues), see Phobius details PF00403: HMA" amino acids 53 to 109 (57 residues), 46.8 bits, see alignment 6.6e-16 TIGR01512: cadmium-translocating P-type ATPase" amino acids 183 to 730 (548 residues), 546.4 bits, see alignment E=9.3e-168 TIGR01525: heavy metal translocating P-type ATPase" amino acids 183 to 728 (546 residues), 602.3 bits, see alignment E=1.6e-184 TIGR01494: HAD ATPase, P-type, family IC" amino acids 206 to 705 (500 residues), 263.3 bits, see alignment E=4.9e-82 PF00122: E1-E2_ATPase" amino acids 234 to 414 (181 residues), 160.6 bits, see alignment E=6e-51 PF00702: Hydrolase" amino acids 433 to 639 (207 residues), 117.7 bits, see alignment E=1.8e-37 PF08282: Hydrolase_3" amino acids 621 to 672 (52 residues), 25.1 bits, see alignment 2.8e-09

Best Hits

Swiss-Prot: 100% identical to ZNTA_ECOLI: Zinc/cadmium/lead-transporting P-type ATPase (zntA) from Escherichia coli (strain K12)

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 100% identity to eco:b3469)

MetaCyc: 100% identical to Zn2+/Cd2+/Pb2+ exporting P-type ATPase (Escherichia coli K-12 substr. MG1655)
3.6.3.3-RXN [EC: 7.2.2.21]; RXN0-5205 [EC: 7.2.2.21, 7.2.2.12]; 7.2.2.- [EC: 7.2.2.21, 7.2.2.12]

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5 or 7.2.2.12 or 7.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P37617 at UniProt or InterPro

Protein Sequence (732 amino acids)

>b3469 zinc, cobalt and lead efflux system (NCBI) (Escherichia coli BW25113)
MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSENVSGTRYSWKVSGMDCA
ACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVESALQKAGYSLRDEQAAEEP
QASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATTLVGLYPIARQALRLIKSGSY
FAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEGWAASRARQGVSALMALKPETAT
RLRKGEREEVAINSLRPGDVIEVAAGGRLPADGKLLSPFASFDESALTGESIPVERATGD
KVPAGATSVDRLVTLEVLSEPGASAIDRILKLIEEAEERRAPIERFIDRFSRIYTPAIMA
VALLVTLVPPLLFAASWQEWIYKGLTLLLIGCPCALVISTPAAITSGLAAAARRGALIKG
GAALEQLGRVTQVAFDKTGTLTVGKPRVTAIHPATGISESELLTLAAAVEQGATHPLAQA
IVREAQVAELAIPTAESQRALVGSGIEAQVNGERVLICAAGKHPADAFTGLINELESAGQ
TVVLVVRNDDVLGVIALQDTLRADAATAISELNALGVKGVILTGDNPRAAAAIAGELGLE
FKAGLLPEDKVKAVTELNQHAPLAMVGDGINDAPAMKAAAIGIAMGSGTDVALETADAAL
THNHLRGLVQMIELARATHANIRQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVL
VTANALRLLRRR