Protein Info for b3384 in Escherichia coli BW25113

Name: trpS
Annotation: tryptophanyl-tRNA synthetase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 334 PF00579: tRNA-synt_1b" amino acids 3 to 285 (283 residues), 296.2 bits, see alignment E=1.3e-92 TIGR00233: tryptophan--tRNA ligase" amino acids 3 to 330 (328 residues), 391.8 bits, see alignment E=1.4e-121

Best Hits

Swiss-Prot: 100% identical to SYW_ECOLI: Tryptophan--tRNA ligase (trpS) from Escherichia coli (strain K12)

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 100% identity to eco:b3384)

MetaCyc: 100% identical to tryptophan--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Tryptophan--tRNA ligase. [EC: 6.1.1.2]; 6.1.1.2 [EC: 6.1.1.2]

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P00954 at UniProt or InterPro

Protein Sequence (334 amino acids)

>b3384 tryptophanyl-tRNA synthetase (NCBI) (Escherichia coli BW25113)
MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQHAITVRQDAQKLRKAT
LDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFGELSRMTQFKDKSARYAEN
INAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDIAQRFNALYGEIFKVPEPFIP
KSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVKKIKRAVTDSDEPPVVRYDVQNK
AGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGEVADAVSGMLTELQERYHRFRNDEA
FLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP