Protein Info for b3359 in Escherichia coli BW25113

Name: argD
Annotation: bifunctional acetylornithine aminotransferase/ succinyldiaminopimelate aminotransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03246: succinylornithine transaminase family
  • amino acids 8 to 404 (397 residues), 680.3 bits, E=8.4e-209
• TIGR00707: transaminase, acetylornithine/succinylornithine family
  • amino acids 17 to 400 (384 residues), 497.7 bits, E=2e-153
• PF00202: Aminotran_3
  • amino acids 26 to 400 (375 residues), 499 bits, E=9.1e-154
• PF00155: Aminotran_1_2
  • amino acids 155 to 338 (184 residues), 26 bits, E=5.5e-10

Best Hits

Swiss-Prot: 100% identical to ARGD_ECOLI: Acetylornithine/succinyldiaminopimelate aminotransferase (argD) from Escherichia coli (strain K12)

KEGG orthology group: K00821, acetylornithine/N-succinyldiaminopimelate aminotransferase [EC: 2.6.1.11 2.6.1.17] (inferred from 100% identity to eco:b3359)

MetaCyc: 100% identical to N-acetylornithine aminotransferase / N-succinyldiaminopimelate aminotransferase (Escherichia coli K-12 substr. MG1655)
Succinyldiaminopimelate transaminase. [EC: 2.6.1.17]; Acetylornithine transaminase. [EC: 2.6.1.17, 2.6.1.11]

Predicted SEED Role

"Acetylornithine aminotransferase (EC 2.6.1.11) / N-succinyl-L,L-diaminopimelate aminotransferase (EC 2.6.1.17)" in subsystem Arginine Biosynthesis extended or Lysine Biosynthesis DAP Pathway (EC 2.6.1.11, EC 2.6.1.17)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• superpathway of arginine and polyamine biosynthesis (16/17 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-ornithine biosynthesis I (5/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.11 or 2.6.1.17

Sequence Analysis Tools

See P18335 at UniProt or InterPro

Protein Sequence (406 amino acids)

>b3359 bifunctional acetylornithine aminotransferase/ succinyldiaminopimelate aminotransferase (NCBI) (Escherichia coli BW25113)
MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEYVDFAGGIAVTALGHCH
PALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERVVFMNSGTEANETAFKLAR
HYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDGFGPKPADIIHVPFNDLHAVK
AVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQHQALLVFDEVQCGMGRTGDLFA
YMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAFHPGSHGSTYGGNPLACAVAGAAFD
IINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSDIRGMGLLIGAELKPQYKGRARDFLYA
GAEAGVMVLNAGPDVMRFAPSLVVEDADIDEGMQRFAHAVAKVVGA